All Phenotypes Tg(Gpx4)#Yana MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gpx4^tm1Yana/Gpx4^tm1Yana Tg(Crx-cre)1Tfur/0 Tg(Gpx4)#Yana/0	involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * DBA/2	MGI:6383173
cn2	Gpx4^tm1Yana/Gpx4^tm1Yana Tg(Gpx4)#Yana/0 Tg(Pgk2-cre)24Shb/0	involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * DBA/2 * ICR	MGI:6383181
cx3	Gpx4^tm1Yana/Gpx4^tm1Yana Tg(Gpx4)#Yana/0	involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * DBA/2 * ICR	MGI:6383180

Allelic Composition	Gpx4^tm1Yana/Gpx4^tm1Yana Tg(Crx-cre)1Tfur/0 Tg(Gpx4)#Yana/0
Genetic Background	involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4^tm1Yana mutation (0 available); any Gpx4 mutation (82 available)
Tg(Crx-cre)1Tfur mutation (1 available)
Tg(Gpx4)#Yana mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

vision/eye

abnormal retina morphology ( J:182776 )

• lipid peroxidation is increased in inner segment photoreceptor cells at P12, and a modest increase is seen in the other layers of the retina including the inner nuclear layer and ganglion cell layer

abnormal photoreceptor connecting cilium morphology ( J:182776 )

• 80% decrease in the number of connecting cilia in photoreceptor cells

abnormal photoreceptor inner segment morphology ( J:182776 )

• mitochondrial area in inner segment of photoreceptor cells is decreased indicating a decrease in mitochondrial biomass at P11

abnormal photoreceptor outer segment morphology ( J:182776 )

• the outer segment layer is very thin

disorganized photoreceptor outer segment ( J:182776 )

• the outer segment is disorganized and consists of cell membranes that branch and form a large web-like network structure without apparent disc rim and an abnormally wide intra- and inter-outer segment membrane space

thin retina inner nuclear layer ( J:182776 )

• milder thinning or degeneration of the inner nuclear layer

retina outer nuclear layer degeneration ( J:182776 )

• rapid loss of the outer nuclear layer

• an increasing number of TUNEL+ cells are seen in the outer nuclear layer after P12

retina degeneration ( J:182776 )

• mice show rapid and massive retinal degeneration after P12 with none of the photoreceptor cells retained by P21

• cone photoreceptor cells are lost earlier than rod photoreceptor cells

nervous system

abnormal photoreceptor connecting cilium morphology ( J:182776 )

• 80% decrease in the number of connecting cilia in photoreceptor cells

abnormal photoreceptor inner segment morphology ( J:182776 )

• mitochondrial area in inner segment of photoreceptor cells is decreased indicating a decrease in mitochondrial biomass at P11

abnormal photoreceptor outer segment morphology ( J:182776 )

• the outer segment layer is very thin

disorganized photoreceptor outer segment ( J:182776 )

cellular

abnormal photoreceptor connecting cilium morphology ( J:182776 )

• 80% decrease in the number of connecting cilia in photoreceptor cells

decreased mitochondrial DNA content ( J:182776 )

• mitochondrial DNA replication in the whole retina is lower

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

decreased body weight ( J:156326 )

• 9 week old mice show a decrease in total body weight

reproductive system

abnormal male germ cell morphology ( J:156326 )

• mice show an increase in diploid germ cells whereas cells with haploid DNA content are reduced

• elevation of intracellular hydroperoxide in germ cells

abnormal sperm mitochondrial morphology ( J:156326 )

• the internal membrane structure of the spermatozoa mitochondria is irregular, with disappearance of cristae-like folds and formation of electron-dense central clumps

• however, the outer mitochondrial membranes are normal

hairpin sperm flagellum ( J:156326 )

• epididymal spermatozoa exhibit severe flagellar defects, with flagellum folded at the midpiece-principal junction into a sharp hairpin configuration

• testicular spermatozoa are fully formed and have extended flagellum, however caput spermatozoa show abnormal flagellar bending in the posterior midpiece of the flagellum

decreased male germ cell number ( J:156326 )

• in 70% of seminiferous tubules, little or no spermatocytes, spermatids, or sperm are seen

• only spermatogonia are seen among germinal cells in damaged seminiferous tubules, indicating that germinal cells die before postnatal mitosis but not before meiosis

• mice show more seminiferous tubules with activated caspase 3+ spermatocytes, indicating germ cell death

oligozoospermia ( J:156326 )

• spermatozoa number in epididymal semen is decreased

small testis ( J:156326 )

decreased testis weight ( J:156326 )

male infertility ( J:156326 )

• males are infertile

• spermatozoa show no evidence of fertilization of wild-type eggs in vitro

abnormal sperm physiology ( J:156326 )

• mitochondrial membrane potential of cauda spermatozoa is decreased after 2 hours in vitro incubation in TYH medium but is normally maintained when in vitro incubation is omitted

• mitochondria of cauda spermatozoa show a reduction in the active respiration state and a decrease in the respiratory efficiency

asthenozoospermia ( J:156326 )

• forward motility of spermatozoa is reduced to 24% of controls

• hairpin configuration spermatozoa frequently show weak flagellar beating particularly at the principal segment

cellular

abnormal male germ cell morphology ( J:156326 )

• mice show an increase in diploid germ cells whereas cells with haploid DNA content are reduced

• elevation of intracellular hydroperoxide in germ cells

abnormal sperm mitochondrial morphology ( J:156326 )

• the internal membrane structure of the spermatozoa mitochondria is irregular, with disappearance of cristae-like folds and formation of electron-dense central clumps

• however, the outer mitochondrial membranes are normal

hairpin sperm flagellum ( J:156326 )

• epididymal spermatozoa exhibit severe flagellar defects, with flagellum folded at the midpiece-principal junction into a sharp hairpin configuration

• testicular spermatozoa are fully formed and have extended flagellum, however caput spermatozoa show abnormal flagellar bending in the posterior midpiece of the flagellum

decreased male germ cell number ( J:156326 )

• in 70% of seminiferous tubules, little or no spermatocytes, spermatids, or sperm are seen

• only spermatogonia are seen among germinal cells in damaged seminiferous tubules, indicating that germinal cells die before postnatal mitosis but not before meiosis

• mice show more seminiferous tubules with activated caspase 3+ spermatocytes, indicating germ cell death

oligozoospermia ( J:156326 )

• spermatozoa number in epididymal semen is decreased

abnormal mitochondrial crista morphology ( J:156326 )

• the internal membrane structure of the spermatozoa mitochondria is irregular, with disappearance of cristae-like folds and formation of electron-dense central clumps

• however, the outer mitochondrial membranes are normal

asthenozoospermia ( J:156326 )

• forward motility of spermatozoa is reduced to 24% of controls

• hairpin configuration spermatozoa frequently show weak flagellar beating particularly at the principal segment

abnormal mitochondrial physiology ( J:156326 )

• mitochondrial membrane potential of cauda spermatozoa is decreased after 2 hours in vitro incubation in TYH medium but is normally maintained when in vitro incubation is omitted

• mitochondria of cauda spermatozoa show a reduction in the active respiration state and a decrease in the respiratory efficiency

endocrine/exocrine glands

small testis ( J:156326 )

decreased testis weight ( J:156326 )

Allelic Composition	Gpx4^tm1Yana/Gpx4^tm1Yana Tg(Gpx4)#Yana/0
Genetic Background	involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * DBA/2 * ICR

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4^tm1Yana mutation (0 available); any Gpx4 mutation (82 available)
Tg(Gpx4)#Yana mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:156326 )

• mice are viable and fertile and live for at least more than 1 year, indicating rescue of the embryonic lethality seen in Gpx4 knock-out mice

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