reproductive system
N |
• relative weights of testes and cauda epididymis are not significantly reduced relative to those in wild-type males
• seminiferous tubule morphology is normal
|
• Mito Red immunostaining of cauda epididymal sperm showed various abnormalities ranging from an abnormally thick midpiece, a slightly tapered bent thick neck, and cytoplasmic droplets with amorphous sperm heads
• ~55% of Mito Red-stained sperm show flagellar defects
|
• a slightly tapered bent thick neck is observed
|
• thick sperm midpieces with distal cytoplasmic droplets are observed
• however, mature sperm flagella show normal F-actin organization in the midpiece
|
• plasma membrane does not cover the mitochondrial sheath uniformly
• a fibrous sheet containing more than one axoneme-mitochondrial complex is observed
|
• disorganized or vacuolated mitochondria are observed
|
necrospermia
(
J:320651
)
• Eosin and Nigrosine (E&N) staining revealed that the percentage of viable sperm is borderline (~40%) relative to that in heterozygous and wild-type males (60-80%)
• hypo-osmotic swelling test (where intact live sperm show a swelling/curling of the tail) revealed that the percentage of hypo-osmotic reactive sperm is also borderline
|
• cauda epididymal sperm count is significantly lower than that in heterozygous and wild-type males
|
• DAPI-stained sperm cells exhibit abnormal head shape with alterations in area, circularity, length of hook, bounding width, and regularity
|
• 51-56% of mature epididymal spermatozoa exhibit malformed/fragmented acrosomes
|
• acrosomal biogenesis is impaired at the Golgi phase: PNA staining of adult testis sections is scattered in Golgi-phase spermatids, suggesting a less uniform acrosomal compartment; TEM analysis confirmed the presence a loosely clustered granular structure
• in the cap phase, cap structures are more unevenly distributed in round spermatids; TEM showed a bulgier and detached cap
• in the acrosomal phase, acrosomal content fails to form an arrow-like shape in elongating spermatids; TEM showed a malformed developing acrosome covering
• in the maturation phase, TEM revealed a malformed acrosomal structure
• arrested acrosome formation is due to failure of proacrosomal granule formation and fusion associated with deregulation of autophagy master regulators (Trim27, AMPK, mTOR, Atg2a, LC3B, and SQSTM1)
|
• failure of proacrosomal granule formation and fusion
|
• 70-80% of sperm show irregular/round head morphology
|
• in the cap phase, the acrosomal vesicle fails to form a continuous cap-like structure and presents as a granule detached from the acroplaxome
|
• DAPI-stained sperm cells show amorphous nuclear morphology
|
• CASA analysis revealed a significant reduction in progressive sperm motility
|
• CASA analysis revealed a significant reduction in total sperm motility
• however, curvilinear velocity (VCL), straight-line velocity (VSL), and average path velocity (VAP) are not significantly reduced
|
• spermiogenesis is impaired as shown by defects in acrosome biogenesis, autophagy, manchette development and mitochondrial organization
• mature sperm display a cytoplasm removal defect
|
• round spermatids show abnormal manchette development
• defects in manchette development are more prominent in step 9-13 spermatids, leading to an amorphous sperm head shape
|
• mating of homozygous males with wild-type females results in an average litter of 2 relative to 7.9 for heterozygous males and 8.2 for wild-type males
|
• adult males are subfertile
• however, mating behavior is normal as vaginal plugs are clearly observed
|
• in vitro, the rate of A23187-induced acrosome reaction is significantly reduced, with only 37.5% of cauda epididymal sperm undergoing acrosome exocytosis versus 60% of sperm in wild-type males
|
cellular
• Mito Red immunostaining of cauda epididymal sperm showed various abnormalities ranging from an abnormally thick midpiece, a slightly tapered bent thick neck, and cytoplasmic droplets with amorphous sperm heads
• ~55% of Mito Red-stained sperm show flagellar defects
|
• a slightly tapered bent thick neck is observed
|
• thick sperm midpieces with distal cytoplasmic droplets are observed
• however, mature sperm flagella show normal F-actin organization in the midpiece
|
• plasma membrane does not cover the mitochondrial sheath uniformly
• a fibrous sheet containing more than one axoneme-mitochondrial complex is observed
|
• disorganized or vacuolated mitochondria are observed
|
necrospermia
(
J:320651
)
• Eosin and Nigrosine (E&N) staining revealed that the percentage of viable sperm is borderline (~40%) relative to that in heterozygous and wild-type males (60-80%)
• hypo-osmotic swelling test (where intact live sperm show a swelling/curling of the tail) revealed that the percentage of hypo-osmotic reactive sperm is also borderline
|
• cauda epididymal sperm count is significantly lower than that in heterozygous and wild-type males
|
• DAPI-stained sperm cells exhibit abnormal head shape with alterations in area, circularity, length of hook, bounding width, and regularity
|
• 51-56% of mature epididymal spermatozoa exhibit malformed/fragmented acrosomes
|
• acrosomal biogenesis is impaired at the Golgi phase: PNA staining of adult testis sections is scattered in Golgi-phase spermatids, suggesting a less uniform acrosomal compartment; TEM analysis confirmed the presence a loosely clustered granular structure
• in the cap phase, cap structures are more unevenly distributed in round spermatids; TEM showed a bulgier and detached cap
• in the acrosomal phase, acrosomal content fails to form an arrow-like shape in elongating spermatids; TEM showed a malformed developing acrosome covering
• in the maturation phase, TEM revealed a malformed acrosomal structure
• arrested acrosome formation is due to failure of proacrosomal granule formation and fusion associated with deregulation of autophagy master regulators (Trim27, AMPK, mTOR, Atg2a, LC3B, and SQSTM1)
|
• failure of proacrosomal granule formation and fusion
|
• 70-80% of sperm show irregular/round head morphology
|
• in the cap phase, the acrosomal vesicle fails to form a continuous cap-like structure and presents as a granule detached from the acroplaxome
|
• DAPI-stained sperm cells show amorphous nuclear morphology
|
• round spermatids show abnormal manchette development
• defects in manchette development are more prominent in step 9-13 spermatids, leading to an amorphous sperm head shape
|
• spermatids exhibit defects and disorganization in cis- and trans-Golgi network
• disruption of the Golgi sub-domains causes abnormal formation of Golgi-derived proacrosomal vesicles leading to acrosome malformation
|
• mTOR signaling is activated while AMPK is suppressed, leading to accumulation of LC3B and SQSTM1 proteins in the testes, indicating disruption of the autophagic flux
|
• CASA analysis revealed a significant reduction in progressive sperm motility
|
• CASA analysis revealed a significant reduction in total sperm motility
• however, curvilinear velocity (VCL), straight-line velocity (VSL), and average path velocity (VAP) are not significantly reduced
|
• acrosome development is impaired due to defective transport of vesicles released from Golgi complexes
|
homeostasis/metabolism
• mTOR signaling is activated while AMPK is suppressed, leading to accumulation of LC3B and SQSTM1 proteins in the testes, indicating disruption of the autophagic flux
|
• LC3B, P62/SQSTM1, mTOR and p-mTOR protein levels are increased in testes, indicating inhibition in autophagy
• actin-related protein T3 (ACTRT3, aka ARPM1) is absent in the nuclear fraction of testes and sperm, suggesting that destabilization of the PFN3-ARPM1 complex results in the degradation of ARPM1
• testes show increased protein levels of essential actin regulatory proteins, cofilin-1 (CFL1), cofilin-2 (CFL2), and destrin (DSTN, aka actin depolymerizing factor or ADF)
|