Phenotypes associated with this allele

• Allele Symbol: Isy1^em1(IMPC)J
• Allele Name: endonuclease-mediated mutation 1, Jackson
• Allele ID: MGI:6384859
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Isy1^em1(IMPC)J/Isy1^em1(IMPC)J	C57BL/6NJ-Isy1^em1(IMPC)J/Mmjax	MGI:6696783
ht2	Isy1^em1(IMPC)J/Isy1^+	C57BL/6NJ-Isy1^em1(IMPC)J/Mmjax	MGI:7317007

Genotype
MGI:6696783

hm1

• Allelic Composition: Isy1^em1(IMPC)J/Isy1^em1(IMPC)J
• Genetic Background: C57BL/6NJ-Isy1^em1(IMPC)J/Mmjax

Data Sources

IMPC Data for Isy1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Isy1^em1(IMPC)J/Isy1^em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E3.5 that appear as dying morulae but no embryos at E7.5. Embryos fail to hatch from the zona pellucida and to form outgrowths in vitro.

embryo

failure of blastocyst to hatch from the zona pellucida ( J:279207 )

• in vitro outgrowth assays show that embryos fail to hatch from the zona pellucida after 3 days in culture

abnormal morula morphology ( J:279207 )

• embryos recovered at E3.5 appear as dying morulae

mortality/aging

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - JAX

preweaning lethality, complete penetrance ( J:211773 )

IMPC - JAX

embryonic lethality before implantation, complete penetrance ( J:279207 )

• homozygous embryos are recovered at E3.5 (11% versus expected 25%, n=44) and appear as dying morulae; no homozygous embryos or excess resorption sites are observed at E7.5

reproductive system

failure of embryo implantation ( J:279207 )

• in vitro, embryos fail to hatch and are dead after 3 days in culture, never forming outgrowths

Genotype
MGI:7317007

ht2

• Allelic Composition: Isy1^em1(IMPC)J/Isy1⁺
• Genetic Background: C57BL/6NJ-Isy1^em1(IMPC)J/Mmjax

Data Sources

IMPC Data for Isy1

hematopoietic system

decreased leukocyte cell number ( J:211773 )

IMPC - JAX

immune system

decreased leukocyte cell number ( J:211773 )

IMPC - JAX