Phenotypes associated with this allele

• Allele Symbol: Thoc3^em1(IMPC)J
• Allele Name: endonuclease-mediated mutation 1, Jackson
• Allele ID: MGI:6388620
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Thoc3^em1(IMPC)J/Thoc3^em1(IMPC)J	C57BL/6NJ-Thoc3^em1(IMPC)J/Mmjax	MGI:6696937
ht2	Thoc3^em1(IMPC)J/Thoc3^+	C57BL/6NJ-Thoc3^em1(IMPC)J/Mmjax	MGI:7267108

Genotype
MGI:6696937

hm1

• Allelic Composition: Thoc3^em1(IMPC)J/Thoc3^em1(IMPC)J
• Genetic Background: C57BL/6NJ-Thoc3^em1(IMPC)J/Mmjax

Data Sources

IMPC Data for Thoc3

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Thoc3^em1(IMPC)J/Thoc3^em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E3.5 as uncompacted morula but not at E7.5. Embryos in vitro hatch from the zona pellucida but form atypical outgrowths with an abnormal inner cell mass and few trophectoderm cells.

embryo

failure of morula compaction ( J:279207 )

• embryos recovered at E3.5 appear as uncompacted morulae

abnormal inner cell mass morphology ( J:279207 )

• in vitro outgrowth assays show an irregular ICM after 3 days in culture

abnormal trophectoderm morphology ( J:279207 )

• in vitro, few trophectoderm (TE) cells are observed after 3 days in culture

mortality/aging

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - JAX

preweaning lethality, complete penetrance ( J:211773 )

IMPC - JAX

embryonic lethality prior to organogenesis, complete penetrance ( J:279207 )

• homozygous embryos are recovered at nearly normal Mendelian ratios at E3.5 (20% versus expected 25%) and appear as uncompacted morulae; no homozygous embryos are recovered at E7.5

• however, excess resorption sites suggest that embryos elicit a decidual response in vivo

reproductive system

abnormal embryo implantation ( J:279207 )

• in vitro, embryos hatch from the zona pellucida but form atypical outgrowths after 3 days in culture

Genotype
MGI:7267108

ht2

• Allelic Composition: Thoc3^em1(IMPC)J/Thoc3⁺
• Genetic Background: C57BL/6NJ-Thoc3^em1(IMPC)J/Mmjax

Data Sources

IMPC Data for Thoc3

behavior/neurological

increased grip strength ( J:211773 )

♀

IMPC - JAX

hearing/vestibular/ear

abnormal auditory brainstem response ( J:211773 )

IMPC - JAX