Phenotypes associated with this allele

• Allele Symbol: Klk6^tm1Gsot
• Allele Name: targeted mutation 1, Georgia Sotiropoulou
• Allele ID: MGI:6444221
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Klk6^tm1Gsot/Klk6^tm1Gsot	involves: C57BL/6	MGI:6444240
ht2	Klk6^tm1Gsot/Klk6^+	involves: C57BL/6	MGI:6444241
cx3	Klk6^tm1Gsot/Klk6^tm1Gsot Spink5^tm1Drh/Spink5^tm1Drh	involves: 129P2/OlaHsd * C57BL/6	MGI:6444340

Genotype
MGI:6444240

hm1

• Allelic Composition: Klk6^tm1Gsot/Klk6^tm1Gsot
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

decreased incidence of induced tumors ( J:270051 )

• mice transplanted subcutaneously with PDVC57 mouse skin squamous carcinoma cells show delayed onset of tumor formation compared to wild-type mice, resulting in decelerated growth and reduced numbers of skin tumors

decreased incidence of tumors by chemical induction ( J:270051 )

• mice treated with DMBA/TPA to induce tumorigenesis show a delay in onset of tumors compared to wild-type mice

• DMBA/TPA induced-tumors are mostly papillomas whereas wild-type tumors progress to squamous cell carcinomas

immune system

abnormal cytokine level ( J:270051 )

• DMBA/TPA-induced papillomas show suppressed expression of inflammatory cytokines, including Tslp, Il-17, Il-23, Il-1b, and Tnf-alpha

• PDVC57 tumors show reduced expression of Il-1b, Il-17, and Tnf-alpha

decreased inflammatory response ( J:270051 )

• tumors from DMBA/TPA-induced mice show decreased numbers of infiltrating macrophages, polymorphonuclear cells, and lymphocytes compared to wild-type mice

decreased susceptibility to chemically induced skin inflammation ( J:270051 )

• mice exhibit suppressed TPA-induced skin inflammation compared to wild-type mice, showing decreased expression of cytokines Tnf-alpha, Tslp, IL-1b, Il-17, Il-18, and Il-23 and reduced thickness of the epidermis

homeostasis/metabolism

abnormal cytokine level ( J:270051 )

• DMBA/TPA-induced papillomas show suppressed expression of inflammatory cytokines, including Tslp, Il-17, Il-23, Il-1b, and Tnf-alpha

• PDVC57 tumors show reduced expression of Il-1b, Il-17, and Tnf-alpha

decreased incidence of tumors by chemical induction ( J:270051 )

• mice treated with DMBA/TPA to induce tumorigenesis show a delay in onset of tumors compared to wild-type mice

• DMBA/TPA induced-tumors are mostly papillomas whereas wild-type tumors progress to squamous cell carcinomas

integument

abnormal skin physiology ( J:270051 )

• hypoproliferative epidermis

• however, epidermis shows normal differentiation patterns and whiskers appear normal

decreased susceptibility to chemically induced skin inflammation ( J:270051 )

• mice exhibit suppressed TPA-induced skin inflammation compared to wild-type mice, showing decreased expression of cytokines Tnf-alpha, Tslp, IL-1b, Il-17, Il-18, and Il-23 and reduced thickness of the epidermis

Genotype
MGI:6444241

ht2

• Allelic Composition: Klk6^tm1Gsot/Klk6⁺
• Genetic Background: involves: C57BL/6

neoplasm

decreased incidence of induced tumors ( J:270051 )

• mice transplanted subcutaneously with PDVC57 mouse skin squamous carcinoma cells show delayed onset of tumor formation compared to wild-type mice, resulting in decelerated growth and reduced numbers of skin tumors

decreased incidence of tumors by chemical induction ( J:270051 )

• mice treated with DMBA/TPA to induce tumorigenesis show a delay in onset of tumors compared to wild-type mice

• DMBA/TPA induced-tumors are mostly papillomas whereas wild-type tumors progress to squamous cell carcinomas

immune system

decreased inflammatory response ( J:270051 )

• tumors from DMBA/TPA-induced mice show decreased numbers of infiltrating macrophages, polymorphonuclear cells, and lymphocytes compared to wild-type mice

decreased susceptibility to chemically induced skin inflammation ( J:270051 )

• mice treated with TPA to induce skin inflammation show reduced epidermis thickness compared to wild-type mice

homeostasis/metabolism

decreased incidence of tumors by chemical induction ( J:270051 )

• mice treated with DMBA/TPA to induce tumorigenesis show a delay in onset of tumors compared to wild-type mice

• DMBA/TPA induced-tumors are mostly papillomas whereas wild-type tumors progress to squamous cell carcinomas

integument

decreased susceptibility to chemically induced skin inflammation ( J:270051 )

• mice treated with TPA to induce skin inflammation show reduced epidermis thickness compared to wild-type mice

Genotype
MGI:6444340

cx3

• Allelic Composition: Klk6^tm1Gsot/Klk6^tm1Gsot; Spink5^tm1Drh/Spink5^tm1Drh
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

neonatal lethality, complete penetrance ( J:289877 )

• mice survive up to 5 hours after birth and die from severe epidermal barrier defect

integument

integument phenotype ( J:289877 )

N • mice exhibit normal epidermal differentiation

impaired skin barrier function ( J:289877 )

• severe epidermal barrier defect, with mice losing water with the same rate at single Spink5^tm1Drh homozygotes

abnormal vibrissa morphology ( J:289877 )

• vibrissae defects

abnormal skin morphology ( J:289877 )

• mice display a similar skin phenotype as single Spink5^tm1Drh homozygotes

abnormal epidermis stratum corneum morphology ( J:289877 )

• stratum corneum detachment

epidermal desquamation ( J:289877 )

• severe desquamation

immune system

abnormal cytokine level ( J:289877 )

• mice show reduced levels of inflammatory cytokine expression compared to single Spink5^tm1Drh homozygotes, including Tslp, Tnf-alpha, Il-16, Il-6, Il-17a, Il-13, and Il-4

decreased inflammatory response ( J:289877 )

• mice exhibit reduced epidermal inflammation seen in single Spink5^tm1Drh homozygotes, showing reduced mast cell infiltration in the epidermis and reduced levels of inflammatory cytokine expression

homeostasis/metabolism

impaired skin barrier function ( J:289877 )

• severe epidermal barrier defect, with mice losing water with the same rate at single Spink5^tm1Drh homozygotes

abnormal cytokine level ( J:289877 )

• mice show reduced levels of inflammatory cytokine expression compared to single Spink5^tm1Drh homozygotes, including Tslp, Tnf-alpha, Il-16, Il-6, Il-17a, Il-13, and Il-4