Phenotypes associated with this allele

• Allele Symbol: Pfkfb3^tm1.1Pec
• Allele Name: targeted mutation 1.1, Peter Carmeliet
• Allele ID: MGI:6470752
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pfkfb3^tm1.1Pec/Pfkfb3^tm1.1Pec Tg(Pdgfb-icre/ERT2,-EGFP)1Frut/0	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NCrl * CBA	MGI:6470897

Genotype
MGI:6470897

cn1

• Allelic Composition: Pfkfb3^tm1.1Pec/Pfkfb3^tm1.1Pec; Tg(Pdgfb-icre/ERT2,-EGFP)1Frut/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NCrl * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

decreased vascular endothelial cell number ( J:296552 )

• reduced proliferation rate after tamoxifen administration and 7 days after hind-limb ischemia (HLI) induction

abnormal capillary morphology ( J:296552 )

• reduced intercapillary anastomosis in calf muscles after tamoxifen administration and 28 days after hind-limb ischemia (HLI) induction

abnormal vascular development ( J:296552 )

• impaired hind-limb ischemia (HLI)-induced revascularization after tamoxifen administration and 4 weeks after HLI-induction

• lower vascular density after tamoxifen administration and 7 days after hind-limb ischemia (HLI) induction

muscle

decreased skeletal muscle fiber diameter ( J:296552 )

• in calf muscles after tamoxifen administration and 28 days after hind-limb ischemia (HLI) induction

skeletal muscle necrosis ( J:296552 )

• in calf muscles after tamoxifen administration and 28 days after hind-limb ischemia (HLI) induction

• same muscle damage as wildtype in calf muscles after tamoxifen administration and 1 day after hind-limb ischemia (HLI) induction

hematopoietic system

abnormal macrophage morphology ( J:296552 )

• impaired polarization toward M2-like phenotype in calf muscles after tamoxifen administration and 3 days after hind-limb ischemia (HLI) induction

abnormal macrophage physiology ( J:296552 )

• impaired transition from M1-like to M2-like phenotype in calf muscles after tamoxifen administration and 3 days after hind-limb ischemia (HLI) induction

homeostasis/metabolism

abnormal homeostasis ( J:296552 )

• lower lactate levels in conditioned medium of calf muscle endothelial cells isolated after tamoxifen administration and hind-limb ischemia (HLI) induction

• virtually no increase in lactate levels in calf muscles after tamoxifen administration and 12h after hind-limb ischemia (HLI) induction

decreased circulating lactate level ( J:296552 )

• after tamoxifen administration and 12h after hind-limb ischemia (HLI) induction

hypoxia ( J:296552 )

• extensive and widespread in calf muscles after tamoxifen administration and 3 days after hind-limb ischemia (HLI) induction

decreased glycogen catabolism rate ( J:296552 )

• reduced glycolytic flux in calf muscle endothelial cells 10 days after tamoxifen administration

• lower extracellular acidification rate (ECAR) flux in calf muscle endothelial cells 10 days after tamoxifen administration

abnormal pH regulation ( J:296552 )

• lower extracellular acidification rate (ECAR) flux in calf muscle endothelial cells 10 days after tamoxifen administration

abnormal carbohydrate metabolism ( J:296552 )

• reduced glycolytic flux in calf muscle endothelial cells 10 days after tamoxifen administration

• lower extracellular acidification rate (ECAR) flux in calf muscle endothelial cells 10 days after tamoxifen administration

immune system

abnormal macrophage morphology ( J:296552 )

• impaired polarization toward M2-like phenotype in calf muscles after tamoxifen administration and 3 days after hind-limb ischemia (HLI) induction

abnormal macrophage physiology ( J:296552 )

• impaired transition from M1-like to M2-like phenotype in calf muscles after tamoxifen administration and 3 days after hind-limb ischemia (HLI) induction

cellular

cellular phenotype ( J:296552 )

N • normal apoptosis rate of calf muscle endothelial cells after tamoxifen administration and 7 days after hind-limb ischemia (HLI) induction

gangrene ( J:296552 )

• higher incidence of necrotic toes after tamoxifen administration and 4 weeks after hind-limb ischemia (HLI) induction