Phenotypes associated with this allele

• Allele Symbol: St18^{tm1c(KOMP)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:6489608
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Lyz2^tm1(cre)Ifo/Lyz2^+ St18^tm1c(KOMP)Wtsi/St18^tm1c(KOMP)Wtsi	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6N	MGI:6489910
cn2	St18^tm1c(KOMP)Wtsi/St18^tm1c(KOMP)Wtsi Tg(Vil1-cre)20Syr/0	involves: C57BL/6 * C57BL/6N * DBA/2	MGI:6489912
cx3	Casp1^tm1Flv/Casp1^tm1Flv St18^tm1c(KOMP)Wtsi/St18^tm1c(KOMP)Wtsi	involves: C57BL/6 * C57BL/6N * DBA/2	MGI:6489911

Genotype
MGI:6489910

cn1

• Allelic Composition: Lyz2^tm1(cre)Ifo/Lyz2⁺; St18^{tm1c(KOMP)Wtsi}/St18^{tm1c(KOMP)Wtsi}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:298828 )

• reduced survival rates after LPS injection or cecal ligation and puncture (CLP)

immune system

immune system phenotype ( J:298828 )

N • normal M1/M2 macrophage polarization

N • normal NFKB activation and TNFA, IL6 and IL1b production by macrophage colony-stimulating factor (M-CSF)-derived macrophages (MDMs) in response to Toll-like receptor (TLR) and Dectin1 ligands

N • normal serum calcium, TNFA, IL6 and IL12 levels after LPS injection

N • no intestinal inflammation

N • normal TNFA, IL1b and IL17 expression in colon

increased circulating interleukin-1 beta level ( J:298828 )

• elevated serum IL1b levels after LPS injection

increased inflammatory response ( J:298828 )

• increased immune cell infiltration in liver and kidney and, to a lower extent, brain and lung after LPS injection

• increased intestinal inflammation after oral DSS administration

growth/size/body

slow postnatal weight gain ( J:298828 )

• significantly decreased body weight at age 10-15 weeks

increased susceptibility to weight loss ( J:298828 )

• more severe weight loss after oral DSS administration

digestive/alimentary system

diarrhea ( J:298828 )

• increased diarrhea after oral DSS administration

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:298828 )

N • normal serum IGF1 level

increased circulating interleukin-1 beta level ( J:298828 )

• elevated serum IL1b levels after LPS injection

cardiovascular system

abnormal retina blood vessel morphology ( J:298828 )

• increased vessel length and vascular hyperplasia at age P5

increased vascular permeability ( J:298828 )

• vascular leakage in brain, lung, kidney and liver after LPS injection

• vascular leakage in intestines with or without oral DSS administration

vision/eye

abnormal retina blood vessel morphology ( J:298828 )

• increased vessel length and vascular hyperplasia at age P5

Genotype
MGI:6489912

cn2

• Allelic Composition: St18^{tm1c(KOMP)Wtsi}/St18^{tm1c(KOMP)Wtsi}; Tg(Vil1-cre)20Syr/0
• Genetic Background: involves: C57BL/6 * C57BL/6N * DBA/2

growth/size/body

growth/size/body region phenotype ( J:298828 )

N • normal susceptibility to weight loss after oral DSS administration

mortality/aging

mortality/aging ( J:298828 )

N • normal sensitivity to DSS-induced colitis (diarrhea, weight loss, intestinal inflammation, vascular leakage in intestines)

Genotype
MGI:6489911

cx3

• Allelic Composition: Casp1^tm1Flv/Casp1^tm1Flv; St18^{tm1c(KOMP)Wtsi}/St18^{tm1c(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6 * C57BL/6N * DBA/2

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:298828 )

• reduced survival rates after LPS injection