Phenotypes associated with this allele

• Allele Symbol: Alkbh7^tm1.1Klng
• Allele Name: targeted mutation 1.1, Arne Klungland
• Allele ID: MGI:6492321
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Alkbh7^tm1.1Klng/Alkbh7^tm1.1Klng	involves: 129S2/SvPas * C57BL/6J	MGI:6500997

Genotype
MGI:6500997

hm1

• Allelic Composition: Alkbh7^tm1.1Klng/Alkbh7^tm1.1Klng
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:299035 )

N • normal decrease in bone marrow cell number after methylmethanesulfonate (MMS) injection

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:299035 , J:299035 )

♀ • normal sensitivity to methylmethanesulfonate (MMS)-induced mortality (normal LD50 value for MMS injection) (J:299035)

♂ • increased resistance to methylmethanesulfonate (MMS)-induced mortality (increased LD50 value for MMS injection) (J:299035)

adipose tissue

increased body fat mass ( J:198968 , J:198968 , J:198968 )

• extensive fat deposits in abdomen when fed high-fat diet (HFD) (J:198968)

♀ • significant increase when fed SD; more pronounced when fed HFD (J:198968)

♂ • significant increase when fed HFD (J:198968)

increased fat cell size ( J:198968 )

• in white adipose tissue when fed high-fat diet (HFD)

increased abdominal fat pad weight ( J:198968 )

• extensive fat deposits in abdomen when fed high-fat diet (HFD)

increased percent body fat/body weight ( J:198968 )

• when fed high-fat diet (HFD)

behavior/neurological

decreased food intake ( J:198968 )

• hypophagic when fed high-fat diet (HFD)

• normal food intake when fed standard diet (SD)

decreased locomotor activity ( J:198968 )

• 37% reduction in spontaneous activity in open field test

cardiovascular system

increased heart weight ( J:198968 )

• when fed high-fat diet (HFD)

decreased myocardial infarct size ( J:298649 , J:298649 )

♀ • normal ischemia-reperfusion (IR) injury (J:298649)

♂ • reduced ischemia-reperfusion (IR) injury (J:298649)

♂ • normal ischemia-reperfusion (IR) injury in aged males (J:298649)

increased myocardial infarct size ( J:298649 )

♂ • increased ischemia-reperfusion (IR) injury when perfused in absence of glucose

cellular

decreased sensitivity to induced cell death ( J:299035 )

• increased resistance to hydrogen peroxide-, methylmethanesulfonate (MMS)- and methylnitronitrosoguanidine (MNNG)-induced necrosis in mouse embryonic fibroblasts

• normal sensitivity to UV radiation-induced necrosis in mouse embryonic fibroblasts

decreased cellular sensitivity to alkylating agents ( J:299035 )

• increased resistance to methylmethanesulfonate (MMS) and methylnitronitrosoguanidine (MNNG)-induced necrosis in mouse embryonic fibroblasts

decreased cellular sensitivity to methylmethanesulfonate ( J:299035 , J:299035 , J:299035 )

• significantly reduced MMS-induced degeneration of retina ONL (outer nuclear layer) (J:299035)

• increased resistance to MMS-induced necrosis in mouse embryonic fibroblasts (J:299035)

♀ • normal sensitivity to MMS-induced degeneration of cerebellar granule neurons (CGNs) in vivo (J:299035)

♀ • normal sensitivity to MMS-induced mortality (normal LD50 value for MMS injection) (J:299035)

♂ • significantly reduced MMS-induced degeneration of cerebellar granule neurons (CGNs) in vivo (J:299035)

♂ • increased resistance to MMS-induced mortality (increased LD50 value for MMS injection) (J:299035)

decreased cellular sensitivity to hydrogen peroxide ( J:299035 )

• in mouse embryonic fibroblasts

abnormal aerobic respiration ( J:198968 )

• higher mitochondrial respiratory capacity when supplied with pyruvate or glutamate

• normal mitochondrial respiratory capacity when supplied with palmitoyl-carnitine

abnormal mitochondrial physiology ( J:198968 )

• higher respiratory capacity when supplied with pyruvate or glutamate

• normal respiratory capacity when supplied with palmitoyl-carnitine

abnormal fatty acid oxidation ( J:198968 )

• significantly higher levels of long-chain acylcarnitines and short-chain C4-OH and C2 (acetyl-CoA) acylcarnitines and ~50% decreased C3 acylcarnitine level in fed state

• significantly lower levels of acylcarnitines and unchanged (whereas halved in wild-type) C3 and C4 acylcarnitine levels in fasted state

growth/size/body

increased body fat mass ( J:198968 , J:198968 , J:198968 )

• extensive fat deposits in abdomen when fed high-fat diet (HFD) (J:198968)

♀ • significant increase when fed SD; more pronounced when fed HFD (J:198968)

♂ • significant increase when fed HFD (J:198968)

increased heart weight ( J:198968 )

• when fed high-fat diet (HFD)

decreased lean body mass ( J:198968 , J:198968 )

♀ • when fed high-fat diet (HFD) or standard diet (SD) (J:198968)

♂ • normal lean mass when fed high-fat diet (HFD) or standard diet (SD) (J:198968)

obese ( J:198968 )

• significant weight increase from age 1 months (males) or 6 months (females) on regular chow

• increased leptin levels when fed high-fat diet (HFD)

increased susceptibility to diet-induced obesity ( J:198968 )

• reduced resistance to weight gain when fed high-fat diet from week 4 (males) or week 1 (females) on HFD

increased liver weight ( J:198968 )

• when fed high-fat diet (HFD)

liver/biliary system

increased liver weight ( J:198968 )

• when fed high-fat diet (HFD)

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:198968 )

N • normal fasting serum triglyceride and cholesterol levels when fed high-fat diet (HFD)

N • normal circulating ketone body b-hydroxybutyrate levels

N • normal amino acid levels

N • normal glucose tolerance when fed high-fat diet (HFD)

decreased myocardial infarct size ( J:298649 , J:298649 )

♀ • normal ischemia-reperfusion (IR) injury (J:298649)

♂ • reduced ischemia-reperfusion (IR) injury (J:298649)

♂ • normal ischemia-reperfusion (IR) injury in aged males (J:298649)

increased myocardial infarct size ( J:298649 )

♂ • increased ischemia-reperfusion (IR) injury when perfused in absence of glucose

abnormal homeostasis ( J:298649 )

♂ • 25% reduction in perilipin-5 and 31% reduction in fructose-1,6-bisphosphatase 2 (FBP2/PFK2) levels in heart

♂ • reduced levels of antioxidants carnosine and glutathione (GSH) in heart

♂ • faster glycolytic flux in heart

♂ • normal alpha-ketoglutarate, succinate and hydroxyacyl glutathione hydrolase (HAGH, glyoxalase II, GLO2) levels in heart

abnormal fatty acid oxidation ( J:198968 )

• significantly higher levels of long-chain acylcarnitines and short-chain C4-OH and C2 (acetyl-CoA) acylcarnitines and ~50% decreased C3 acylcarnitine level in fed state

• significantly lower levels of acylcarnitines and unchanged (whereas halved in wild-type) C3 and C4 acylcarnitine levels in fasted state

increased susceptibility to diet-induced obesity ( J:198968 )

• reduced resistance to weight gain when fed high-fat diet from week 4 (males) or week 1 (females) on HFD

increased circulating leptin level ( J:198968 )

• when fed high-fat diet (HFD)

abnormal glucose homeostasis ( J:298649 )

♂ • faster glycolytic flux in heart

♂ • normal alpha-ketoglutarate and succinate levels in heart

♂ • 1.6-fold increase in lactoylglutathione lyase (glyoxalase I, GLO1)

♂ • normal alpha-ketoglutarate, succinate and hydroxyacyl glutathione hydrolase (HAGH, glyoxalase II, GLO2) levels in heart

decreased fasting circulating glucose level ( J:198968 )

• after overnight fasting when fed standard diet (SD)

increased circulating insulin level ( J:198968 )

• higher fasting insulin levels when fed high-fat diet (HFD)

increased hepatic glucose production ( J:198968 )

♀ • after overnight fasting when fed high-fat diet (HFD)

increased insulin sensitivity ( J:198968 , J:198968 )

♀ • when fed high-fat diet (HFD) (J:198968)

♂ • normal insulin sensitivity when fed high-fat diet (HFD) (J:198968)

decreased circulating adiponectin level ( J:198968 , J:198968 )

♀ • when fed high-fat diet (HFD) (J:198968)

♂ • normal levels when fed high-fat diet (HFD) (J:198968)

abnormal lipid metabolism ( J:198968 )

• significantly higher levels of long-chain acylcarnitines and short-chain C4-OH and C2 (acetyl-CoA) acylcarnitines and ~50% decreased C3 acylcarnitine level in fed state

• significantly lower levels of acylcarnitines and unchanged (whereas halved in wild-type) C3 and C4 acylcarnitine levels in fasted state