Analysis Tools
mortality/aging
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• mice that escape neonatal death succumb at 6-8 weeks of age
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• although born in normal Mendelian ratios, only 10% of mice (instead of expected 25%) survive to weaning, with newborns dying within the first 2 days of birth
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behavior/neurological
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• at 4 weeks of age, mice exhibit neurological deficits as revealed by SHIRPA testing
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• newborn pups show a significant reduction of reactivity in the nociceptive tail flick test
• however, no difference is observed in the tail suspension test
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respiratory system
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• most pups exhibit difficulties in respiratory activity on the second day after birth
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nervous system
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• ultrastructural analysis of steady-state synapses from hippocampal neurons revealed a ~50% reduction of synaptic vesicles relative to wild-type controls
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• the number of docked/tethered synaptic vesicles per length of active zone is significantly decreased in hippocampal neurons
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growth/size/body
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• at 4 weeks of age
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• at 4 weeks of age
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• mice that escape neonatal death show decreased growth rate
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cellular
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• although the number of synaptic vesicles with a diameter >80 nm is normal under steady-state conditions, analysis of HRP uptake upon depolarization with 50 mM KCl revealed a significant increase in the number of large HRP-positive vesicles (>80 nm) in hippocampal neurons, suggesting that bulk endocytosis is more active in these cells
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