Analysis Tools
growth/size/body
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• under a moderately high-fat (MHF) diet, pair-fed males exhibit a higher rate of increase in fat mass than wild-type controls from 7 to 11 weeks of age, despite a similar body weight gain
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• males either fed a regular diet or an MHF diet from 10 weeks of age gain significantly more body weight than wild-type males; increased body weight gain is also observed in females
• males fed a regular diet exhibit increased body weight at 25 weeks, but not at 9 weeks, of age
• under a regular diet, body length (naso-anal) is normal at 25 weeks of age
• under an MHF-diet, pair-fed males exhibit normal body weight gain from 7 to 11 weeks of age
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• at 25 weeks of age, liver weight is significantly increased in males fed a regular diet
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behavior/neurological
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N |
• males fed an MHF diet from 10 weeks of age exhibit normal diurnal and nocturnal locomotor activity at 18 weeks of age
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• males fed a regular diet show a ~17-20% increase in daily food intake at 8, 11, 16, and 20 weeks of age
• males fed an MHF diet from 10 weeks of age show a 11-19% increase in daily food intake at 11, 15, 19, and 23 weeks of age
• under a regular diet, increased food intake is noted in both male and female mice at 8 weeks of age, i.e. before difference in body weight becomes significant
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polyphagia
(
J:302257
)
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• both male and female mice exhibit hyperphagia (a 10-30% increase in food intake) whether fed a regular or an MHF-diet
• hyperphagia is noted before a significant difference in body weight is observed
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• mice exhibit a significant decrease in the acoustic startle response under some conditions (not shown)
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adipose tissue
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• under a moderately high-fat (MHF) diet, pair-fed males exhibit a higher rate of increase in fat mass than wild-type controls from 7 to 11 weeks of age, despite a similar body weight gain
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• at 25 weeks of age, epididymal fat pad mass is significantly increased in males fed a regular diet
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• at 25 weeks of age, mesenteric fat pad mass is significantly increased in males fed a regular diet
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• at 25 weeks of age, retroperitoneal fat pad mass is significantly increased in males fed a regular diet
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• males fed a regular diet show a significantly increased visceral fat pad weight at 25 weeks of age
• however, body weight and the percentage of fat mass is normal at 9 weeks of age
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homeostasis/metabolism
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N |
• males fed a regular diet exhibit normal plasma glucose, total cholesterol, free fatty acid (FFA), aspartate transaminase (AST) and alanine transaminase (ALT) levels at 9 and 25 weeks of age relative to age-matched wild-type males
• males fed an MHF diet from 10 weeks of age exhibit normal oxygen consumption, respiratory quotient, and rectal temperature at 16-19 weeks of age
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• males fed a regular diet exhibit increased plasma insulin levels at 25 weeks, but not at 9 weeks, of age
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• males fed a regular diet exhibit hyperleptinemia at 25 weeks, but not at 9 weeks, of age
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• males fed a regular diet exhibit increased plasma triglyceride levels at 9 weeks, but not at 25 weeks, of age
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• at 25 weeks of age, liver triglyceride content is significantly increased in males fed a regular diet
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• at 25 weeks of age, hepatic mRNA levels of Srebf1 (aka SREBP1c) are significantly increased in males fed a regular diet
• however, hepatic mRNA levels of other lipogenic genes, Fas and Acaca (aka Acc1), are normal
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liver/biliary system
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• at 25 weeks of age, liver weight is significantly increased in males fed a regular diet
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• at 25 weeks of age, liver triglyceride content is significantly increased in males fed a regular diet
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nervous system
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• at 8 weeks of age, males fed a regular diet exhibit an 18% and 10% reduction, respectively, in mRNA levels of the anorectic molecules Pomc (pro-opiomelanocortin-alpha) and Bdnf (brain derived neurotrophic factor) in the hypothalamus relative to wild-type controls
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