Analysis Tools
digestive/alimentary system
| N |
• no obvious impact on health of mice maintained in specific pathogen-free (SPF) conditions is seen, with mice being fertile, showing no developmental abnormalities or growth impairment, no gross GI tract abnormalities, or intestinal permeability issues
• mice do not develop spontaneous colitis when maintained in SPF conditions for up to 1 year
|
|
• mice with DSS-induced colitis show a greater shortening of the colon than wild-type mice
|
|
• mice exhibit a more aggressive inflammatory response to DSS-induced colitis
• mice are more susceptible than wild-type mice to colonic inflammation upon C. rodentium infection
• mice with C.rodentium-induced colitis show an increase in total weight of the caecum at 13 days post inoculation, suggesting increased inflammatory infiltration
|
|
• mice show an earlier and more pronounced systemic response to DSS-induced colitis compared to wild-type mice, showing greater weight loss and higher mortality rate, a greater shortening of the colon, and more aggressive inflammatory response
• DSS-treated mice show more severe colitis in the colon on days 5 and 9
• mice exhibit increased susceptibility to C. rodentium-induced colitis
• however, mice that survive the acute DSS-induced colitis show restoration of normal colonic architecture by day 21 as in wild-type mice
|
|
• 80% of DSS-treated mice die by day 9 compared 20% of wild-type mice
• 70% of mice with C.rodentium-induced colitis die over the 13-day period compared to no mortality in wild-type mice
|
growth/size/body
|
• DSS-treated mice lose on average 20% of body weight compared to 10% in wild-type mice
• mice with C. rodentium-induced colitis lose approximately 15% of their weight compared to no change in weight for wild-type mice
• mice inoculated with a lower dose of C. rodentium still show weight loss but no mortality
• mice pretreated with antibiotic and orally gavaged with S. typhimurium show a greater loss of body weight than wild-type mice
|
mortality/aging
|
• 80% of DSS-treated mice die by day 9 compared 20% of wild-type mice
• 70% of mice with C.rodentium-induced colitis die over the 13-day period compared to no mortality in wild-type mice
|
|
• 70% of mice with C.rodentium-induced colitis die over the 13-day period compared to no mortality in wild-type mice, however mice inoculated with a lower dose of C. rodentium do not show mortality
• mice pretreated with antibiotic and orally gavaged with S. typhimurium exhibit increased mortality, with 55% of mice succumbing to infection compared to 11% of wild-type mice
|
immune system
|
• mice exhibit a more aggressive inflammatory response to DSS-induced colitis
• mice are more susceptible than wild-type mice to colonic inflammation upon C. rodentium infection
• mice with C.rodentium-induced colitis show an increase in total weight of the caecum at 13 days post inoculation, suggesting increased inflammatory infiltration
|
|
• mice show an earlier and more pronounced systemic response to DSS-induced colitis compared to wild-type mice, showing greater weight loss and higher mortality rate, a greater shortening of the colon, and more aggressive inflammatory response
• DSS-treated mice show more severe colitis in the colon on days 5 and 9
• mice exhibit increased susceptibility to C. rodentium-induced colitis
• however, mice that survive the acute DSS-induced colitis show restoration of normal colonic architecture by day 21 as in wild-type mice
|
|
• 80% of DSS-treated mice die by day 9 compared 20% of wild-type mice
• 70% of mice with C.rodentium-induced colitis die over the 13-day period compared to no mortality in wild-type mice
|
|
• mice with DSS-induced colitis show an elevation in neutrophils compared with wild-type mice after 3 days of DSS exposure, indicating that mice develop an earlier and more aggressive inflammatory response
• however, no difference in number of macrophages, dendritic cells or CD3+ T cells are seen in DSS-treated mice
|
|
• DSS-treated mice exhibit a greater increase in serum IL-1beta levels than in wild-type mice
|
|
• DSS-treated mice exhibit a greater reduction in serum IL-10 levels than in wild-type mice
|
|
• DSS-treated mice exhibit a greater increase in serum IL-6 levels than in wild-type mice
|
|
• mice exhibit increased susceptibility to C. rodentium-induced colitis, showing a greater weight loss and mortality than wild-type mice indicating increased sensitivity to C. rodentium infection
• mice with C.rodentium-induced colitis exhibit systemic infection, showing increased splenic mass and higher numbers of live C. rodentium in the spleens
• mice exhibit increased susceptibility to S. typhimurium infection following pretreatment with antibiotic, with mice showing a greater loss of body weight than in wild-type mice and 55% of mice succumbing to infection compared to 11% of wild-type mice
• however, mice are able to mount an antibody response to C. rodentium infection indicating that mice are able to develop a B cell-mediated adaptive immune response
|
|
• 70% of mice with C.rodentium-induced colitis die over the 13-day period compared to no mortality in wild-type mice, however mice inoculated with a lower dose of C. rodentium do not show mortality
• mice pretreated with antibiotic and orally gavaged with S. typhimurium exhibit increased mortality, with 55% of mice succumbing to infection compared to 11% of wild-type mice
|
hematopoietic system
|
• mice with DSS-induced colitis show an elevation in neutrophils compared with wild-type mice after 3 days of DSS exposure, indicating that mice develop an earlier and more aggressive inflammatory response
• however, no difference in number of macrophages, dendritic cells or CD3+ T cells are seen in DSS-treated mice
|
homeostasis/metabolism
|
• DSS-treated mice exhibit a greater increase in serum IL-1beta levels than in wild-type mice
|
|
• DSS-treated mice exhibit a greater reduction in serum IL-10 levels than in wild-type mice
|
|
• DSS-treated mice exhibit a greater increase in serum IL-6 levels than in wild-type mice
|
