Analysis Tools
skeleton
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• mice show decreased susceptibility to collagen-induced arthritis (CIA), showing reduced incidence of arthritis and reduced severity score, decreased synovial inflammation, decreased pannus formation, and decreased cartilage and bone destruction in joints
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immune system
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• mice show no obvious abnormalities up to 1 year of age under specific pathogen-free conditions and immune cell compositions in lymph nodes, spleen, and bone marrow are normal
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• bone marrow cells treated with granulocyte/macro-phage colony-stimulating factor (GM-CSF) show a lower proportion of the I-A/I-EhiCD11c+ cell population than treated wild-type cells, indicating impaired maturation of dendritic cells
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• the proportion of CD11c+ cell in total lymph node cells is decreased only slightly after CIA induction
• the proportion of I-A/I-E+CD11c+ mature dendritic cells in total CD11c+ dendritic cells is lower after CIA induction
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• mice with CIA show reduced B cell (CD19+) population in inguinal lymph nodes
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• mice with CIA show reduced activated CD4+ T cell (CD44+CD4+) population in inguinal lymph nodes
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• proliferative response of draining lymph node cells to type 2 collagen is suppressed in CIA mice
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• when mutant granulocyte/macrophage colony-stimulating factor-induced dendrtitic cells are cocultured with CD4+ T cells from OT-II mice, T cell proliferative response to OVA is decreased
• however, proliferative responses of T and B cells are similar to wild-type upon stimulation with anti-CD3 and anti-IgM antibodies, respectively and differentiation of CD4+ T cells to regulatory T, Th1 and Th17 cells is normal
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• type 2 collagen-specific serum IgG2a concentrations are reduced in mice with collagen-induced arthritis
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• type 2 collagen-specific serum IgG2b concentrations are reduced in mice with collagen-induced arthritis
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• type 2 collagen-specific serum IgG3 concentrations are reduced in mice with collagen-induced arthritis
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• dendritic cells show impaired antigen-presenting ability following CIA
• T cell responses against type 2 collagen antibodies are greatly reduced in co-culture with mutant dendritic cells, suggesting that antigen-presenting ability of dendritic cells is impaired
• antigen-presenting ability of granulocyte/macrophage colony-stimulating factor (GM-CSF)-induced dendrtitic cells from bone marrow cells is impaired
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• concentration of IFN-gamma in culture supernatant from lymph cell culture is lower after collagen-induced arthritis induction
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• concentration of IL-17A in culture supernatant from lymph cell culture is lower after collagen-induced arthritis induction
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• IL-6 production is reduced in bone marrow neutrophils following stimulation with LPS and zymosan
• IL-6 production is enhanced in bone marrow neutrophils after CpG treatment
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• TNF production is reduced in bone marrow neutrophils upon stimulation with zymosan and M. Tuberculosis
• TNF production is enhanced in bone marrow neutrophils after CpG treatment
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• concentration of TNF in culture supernatant from lymph cell culture is lower after collagen-induced arthritis induction
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• mice show decreased susceptibility to collagen-induced arthritis (CIA), showing reduced incidence of arthritis and reduced severity score, decreased synovial inflammation, decreased pannus formation, and decreased cartilage and bone destruction in joints
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hematopoietic system
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• bone marrow cells treated with granulocyte/macro-phage colony-stimulating factor (GM-CSF) show a lower proportion of the I-A/I-EhiCD11c+ cell population than treated wild-type cells, indicating impaired maturation of dendritic cells
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• the proportion of CD11c+ cell in total lymph node cells is decreased only slightly after CIA induction
• the proportion of I-A/I-E+CD11c+ mature dendritic cells in total CD11c+ dendritic cells is lower after CIA induction
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• mice with CIA show reduced B cell (CD19+) population in inguinal lymph nodes
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• mice with CIA show reduced activated CD4+ T cell (CD44+CD4+) population in inguinal lymph nodes
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• proliferative response of draining lymph node cells to type 2 collagen is suppressed in CIA mice
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• when mutant granulocyte/macrophage colony-stimulating factor-induced dendrtitic cells are cocultured with CD4+ T cells from OT-II mice, T cell proliferative response to OVA is decreased
• however, proliferative responses of T and B cells are similar to wild-type upon stimulation with anti-CD3 and anti-IgM antibodies, respectively and differentiation of CD4+ T cells to regulatory T, Th1 and Th17 cells is normal
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• type 2 collagen-specific serum IgG2a concentrations are reduced in mice with collagen-induced arthritis
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• type 2 collagen-specific serum IgG2b concentrations are reduced in mice with collagen-induced arthritis
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• type 2 collagen-specific serum IgG3 concentrations are reduced in mice with collagen-induced arthritis
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cellular
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• bone marrow cells treated with granulocyte/macro-phage colony-stimulating factor (GM-CSF) show a lower proportion of the I-A/I-EhiCD11c+ cell population than treated wild-type cells, indicating impaired maturation of dendritic cells
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• when mutant granulocyte/macrophage colony-stimulating factor-induced dendrtitic cells are cocultured with CD4+ T cells from OT-II mice, T cell proliferative response to OVA is decreased
• however, proliferative responses of T and B cells are similar to wild-type upon stimulation with anti-CD3 and anti-IgM antibodies, respectively and differentiation of CD4+ T cells to regulatory T, Th1 and Th17 cells is normal
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