Phenotypes associated with this allele

• Allele Symbol: Kif9^em1Osb
• Allele Name: endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• Allele ID: MGI:6511741
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kif9^em1Osb/Kif9^em1Osb	involves: C57BL/6NJcl * DBA/2NJcl	MGI:6827383

Genotype
MGI:6827383

hm1

• Allelic Composition: Kif9^em1Osb/Kif9^em1Osb
• Genetic Background: involves: C57BL/6NJcl * DBA/2NJcl

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:303071 )

♂N • PAS staining of testes revealed no structural abnormalities

♂N • TEM showed no obvious ultrastructural anomalies in the flagellar midpiece or principal piece of spermatozoa within the cauda epididymis

decreased sperm progressive motility ( J:303071 )

♂ • sperm flagella exhibit an asymmetric waveform pattern leading to a circular motion of spermatozoa, unlike control spermatozoa which move linearly

♂ • velocity parameters including average path velocity (VAP), straight line velocity (VSL) and curvilinear velocity (VCL) are all significantly reduced

♂ • however, the % of motile spermatozoa is comparable to than in controls

asthenozoospermia ( J:303071 )

♂ • sperm motility is impaired; tracing of flagellar waveforms shows that most spermatozoa can bend only to the side of the hook (pro-hook), whereas control spermatozoa can bend to both sides (pro-hook and anti-hook)

reduced male fertility ( J:303071 )

♂ • when mated with wild-type females for 2 months, males yield a significantly lower number of litters born per plug relative to heterozygous control males

decreased fertilization frequency ( J:303071 )

♂ • in vitro fertilization (IVF) rate is significantly reduced when cumulus-intact or cumulus-free oocytes are used

♂ • however, IVF rate is normal with zona pellucida-free oocytes, indicating that zona pellucida penetration is partially impaired

impaired sperm penetration of zona pellucida ( J:303071 )

♂ • in vitro, sperm exhibit partially impaired zona pellucida (ZP) penetration

♂ • however, spermatozoa are able to bind to the ZP, and sperm capacitation is normal as determined by tyrosine phosphorylation of sperm proteins

cellular

decreased sperm progressive motility ( J:303071 )

♂ • sperm flagella exhibit an asymmetric waveform pattern leading to a circular motion of spermatozoa, unlike control spermatozoa which move linearly

♂ • velocity parameters including average path velocity (VAP), straight line velocity (VSL) and curvilinear velocity (VCL) are all significantly reduced

♂ • however, the % of motile spermatozoa is comparable to than in controls

asthenozoospermia ( J:303071 )

♂ • sperm motility is impaired; tracing of flagellar waveforms shows that most spermatozoa can bend only to the side of the hook (pro-hook), whereas control spermatozoa can bend to both sides (pro-hook and anti-hook)

nervous system

nervous system phenotype ( J:303071 )

N • mice do NOT exhibit overt hydrocephalus, suggesting that motility of ependymal cilia is normal