All Phenotypes Galnt3<tm1Tok> MGI Mouse

reproductive system phenotype ( J:301856 )

N	• female mice are fertile

oligozoospermia ( J:301856 )

• at 12 weeks of age, the number of epididymal spermatozoa is markedly reduced

teratozoospermia ( J:301856 )

abnormal sperm mitochondrial sheath morphology ( J:301856 )

• in most spermatozoa, the mitochondria sheath is dislocated from the flagellum and found beside the nucleus making a cluster

abnormal sperm head morphology ( J:301856 )

• at 12 weeks of age, all epididymal spermatozoa exhibit abnormal head shapes, classified as round, irregularly round, cylindrical, amorphous, and symplast types

abnormal acrosome assembly ( J:301856 )

• acrosome formation is impaired in round spermatids

• equatorin (an acrosomal N, O-sialoglycoprotein) is present but its MN9 epitope is barely detectable in the acrosomal region

abnormal proacrosomal vesicle fusion ( J:301856 )

• proacrosomal vesicles of various sizes are attached to the nuclear envelope but fail to coalesce and form a single acrosomal vesicle

globozoospermia ( J:301856 )

• ~75% of epididymal spermatozoa display round or irregularly round head morphology

abnormal sperm nucleus morphology ( J:301856 )

• spermatozoa exhibit round or irregularly shaped nuclei rather than elongated nuclei

abnormal spermatid morphology ( J:301856 )

• at 8 weeks of age, spermatids exhibit round or irregularly shaped nuclei

• at 10 weeks of age, elongating spermatids show lack of an acrosome, irregular shape of the nucleus, and vacuolar structures in the head region

• some elongating spermatids show a slightly disorganized mitochondrial arrangement

• many impaired spermatids with an irregularly shaped and condensed nucleus are found not only at the luminal side of seminiferous tubules but also within Sertoli cells

• binding of Vicia villosa agglutinin (VVA) lectin, which preferentially recognizes Tn antigen (GalNAc-O-Ser/Thr) generated by N-acetyl-D-galactosamine (GalNAc) transferase, is reduced in the Golgi stacks and acrosomal vesicles of round spermatids

increased male germ cell apoptosis ( J:301856 )

• at 8 weeks of age, males show a 7.5-fold increase in TUNEL-positive cells in the seminiferous tubules esp. on the luminal side

• many TUNEL-positive cells appear to correspond to impaired spermatids found at the luminal side of seminiferous tubules and within the Sertoli cells

asthenozoospermia ( J:301856 )

• all epididymal spermatozoa exhibit little or no motility

immotile sperm ( J:301856 )

abnormal seminiferous tubule morphology ( J:301856 )

• mucin-type O-glycans (GalNAc-O-Ser/Thr) are drastically reduced in the seminiferous tubules

small testis ( J:301856 )

• at 8 weeks of age, testis size is slightly smaller than that in wild-type males

abnormal spermiogenesis ( J:301856 )

• only a few round-headed spermatozoa with clustered mitochondria are found in the epididymal duct lumen of the proximal caput

male infertility ( J:301856 )

• male mice are consistently infertile