Phenotypes associated with this allele

• Allele Symbol: Gls2^em1Xen
• Allele Name: endonuclease-mediated mutation 1, Taconic Biosciences
• Allele ID: MGI:6695948
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gls2^em1Xen/Gls2^em1Xen	involves: C57BL/6NTac	MGI:6695949

Genotype
MGI:6695949

hm1

• Allelic Composition: Gls2^em1Xen/Gls2^em1Xen
• Genetic Background: involves: C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased circulating glutamine level ( J:271619 )

• fasting glutamine levels are increased

decreased fasting circulating glucose level ( J:271619 )

• mice show a reduction in blood glucose levels after overnight fasting but no change 4 hours after refeeding

• mice fed a 60% high-fat diet show a reduction in fasting glucose levels without significant changes in body weight, insulin, or glucagon

• however, no change in insulin levels are seen

increased circulating glucagon level ( J:271619 )

• fasting plasma glucagon levels are increased

decreased hepatic glucose production ( J:271619 )

• glucagon does not stimulate glucose production in hepatocytes as it does in wild-type hepatocytes

• hepatocytes produce less glucose at baseline than wild-type when stimulated with both lactate and glutamine and are less responsive to glucagon

• however, glucose production in hepatocytes from lactate is similar to wild-type

endocrine/exocrine glands

increased pancreatic alpha cell number ( J:271619 )

• pancreatic islets show alpha cell hyperplasia, with no changes in beta cell mass