Phenotypes associated with this allele

• Allele Symbol: Zfp467^{tm1.1(KOMP)Vlcg}
• Allele Name: targeted mutation 1.1, Velocigene
• Allele ID: MGI:6711762
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zfp467^tm1.1(KOMP)Vlcg/Zfp467^tm1.1(KOMP)Vlcg	B6(Cg)-Zfp467^tm1.1(KOMP)Vlcg	MGI:6711763

Genotype
MGI:6711763

hm1

• Allelic Composition: Zfp467^{tm1.1(KOMP)Vlcg}/Zfp467^{tm1.1(KOMP)Vlcg}
• Genetic Background: B6(Cg)-Zfp467^{tm1.1(KOMP)Vlcg}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

enhanced osteoblast differentiation ( J:302865 )

• markers of osteoblast differentiation are upregulated in calvaria osteoblasts and bone marrow stromal cells

increased osteoclast cell number ( J:302865 )

• osteoclast surface/bone surface is modestly elevated in proximal tibia, with a trend towards an increase in number of osteoclasts/bone perimeter

abnormal osteoclast physiology ( J:302865 )

• markers of osteoblast differentiation and osteoclast activity are increased

abnormal bone marrow morphology ( J:302865 )

• marrow area is decreased

decreased bone marrow adipose tissue amount ( J:302865 )

• 67% reduction in bone marrow adipocyte numbers in tibia at 16 weeks of age

• bone marrow shows a reduced adipocyte volume and adipocyte volume/tissue volume ratio

• however, no difference in adipocyte size in bone marrow adipocyte tissue

• mice fed a high-fat diet exhibit less marrow fat

decreased bone mineral content ( J:302865 )

• total and femoral areal bone mineral content is lower at 8 weeks of age but by 16 weeks, it is similar to wild-type levels

• ovariectomized mice show decreased areal bone mineral content compared to controls

decreased areal bone mineral density ( J:302865 )

• total and femoral areal bone mineral density is lower at 8 weeks of age but by 16 weeks, it is similar to wild-type levels

increased bone volume ( J:302865 )

• bone volume/tissue volume of proximal tibia at 16 weeks of age is increased

abnormal compact bone morphology ( J:302865 )

• cortical porosity is decreased

• however, no difference in cortical tissue mineral density is seen

decreased compact bone area ( J:302865 )

• total area of cortical bone is decreased

increased compact bone mass ( J:302865 )

• increase in cortical bone mass

increased compact bone thickness ( J:302865 )

• cortical area/total area and cortical thickness are increased

abnormal trabecular bone morphology ( J:302865 )

• 35% higher trabecular bone mineral density

• 11% decrease in structural model index

increased trabecular bone volume ( J:302865 )

• 41% higher trabecular bone volume fraction

increased bone trabecula number ( J:302865 )

• trabecular number in proximal tibia at 16 weeks of age is increased

• 21% increase in trabecular number

• mice fed a high-fat diet exhibit higher trabecular number

decreased bone trabecular spacing ( J:302865 )

• 17% decrease in trabecular separation

• trabecular separation in proximal tibia at 16 weeks of age is decreased

• mice fed a high-fat diet exhibit lower trabecular separation

increased trabecular bone connectivity density ( J:302865 )

• 79% greater connectivity density

increased trabecular bone mass ( J:302865 )

decreased trabecular bone thickness ( J:302865 )

• mice fed a high-fat diet exhibit lower trabecular thickness than in controls

increased trabecular bone thickness ( J:302865 )

• trabecular thickness of proximal tibia at 16 weeks of age is increased

increased bone ossification ( J:302865 )

• calvaria osteoblasts and bone marrow stromal cells show an increase in osteogenesis in vitro, showing an increase in matrix mineralization

• mineral surface/bone surface, mineral apposition rate, and bone formation rate/bone surface of proximal tibia are increased

• mice fed a high-fat diet show higher osteogenesis related gene expression

adipose tissue

decreased total body fat amount ( J:302865 )

• 16.2% and 34.4% reduction in total fat at 8 and 16 weeks, respectively

• however, no differences in inguinal, gonadal, and brown adipose tissue weights

• mice are resistant to ovariectomy-induced adiposity but not bone loss, showing decreased areal bone mineral content and femoral bone mineral density and lower inguinal fat weight

abnormal adipose tissue development ( J:302865 )

• calvaria osteoblasts exhibit decreased adipogenesis when grown in culture in osteogenic media

• in vitro ear mesenchymal stem cell adipogenesis cultures show a decrease in lipid droplets

• expression levels of adipogenic related genes is reduced in calvaria osteoblasts but not in bone marrow stromal cells

• mice fed a high-fat diet show reduced adipocyte volume and adipogenesis related gene expression in peripheral and bone marrow fat tissue

• however, in vitro bone marrow stromal cells adipogenic cultures do not show a decrease in lipid droplets

decreased bone marrow adipose tissue amount ( J:302865 )

• 67% reduction in bone marrow adipocyte numbers in tibia at 16 weeks of age

• bone marrow shows a reduced adipocyte volume and adipocyte volume/tissue volume ratio

• however, no difference in adipocyte size in bone marrow adipocyte tissue

• mice fed a high-fat diet exhibit less marrow fat

decreased fat cell size ( J:302865 )

• adipocyte size in inguinal and gonadal fat pads are smaller

decreased inguinal fat pad weight ( J:302865 )

• ovariectomized mice show lower inguinal fat weight than controls

growth/size/body

decreased lean body mass ( J:302865 )

• 5.8% and 6% reduction in lean mass at 8 and 16 weeks, respectively

decreased body size ( J:302865 )

• mice are 7.6% and 12.7% smaller than wild-type mice at 8 and 16 weeks, respectively

hematopoietic system

increased osteoclast cell number ( J:302865 )

• osteoclast surface/bone surface is modestly elevated in proximal tibia, with a trend towards an increase in number of osteoclasts/bone perimeter

abnormal osteoclast physiology ( J:302865 )

• markers of osteoblast differentiation and osteoclast activity are increased

homeostasis/metabolism

improved glucose tolerance ( J:302865 )

• blood glucose levels are lower in the glucose tolerance test indicating improved glucose tolerance

• however, mice show similar blood glucose levels in the insulin tolerance test

immune system

increased osteoclast cell number ( J:302865 )

• osteoclast surface/bone surface is modestly elevated in proximal tibia, with a trend towards an increase in number of osteoclasts/bone perimeter

abnormal osteoclast physiology ( J:302865 )

• markers of osteoblast differentiation and osteoclast activity are increased

cellular

enhanced osteoblast differentiation ( J:302865 )

• markers of osteoblast differentiation are upregulated in calvaria osteoblasts and bone marrow stromal cells