Phenotypes associated with this allele

• Allele Symbol: Zcchc8^em1Sgao
• Allele Name: endonuclease-mediated mutation 1, Shaorong Gao
• Allele ID: MGI:6711985
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zcchc8^em1Sgao/Zcchc8^em1Sgao	involves: C57BL/6 * DBA/2	MGI:6711990

Genotype
MGI:6711990

hm1

• Allelic Composition: Zcchc8^em1Sgao/Zcchc8^em1Sgao
• Genetic Background: involves: C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased survivor rate ( J:297024 )

• mice show lower survival rate in the first 3 months after birth

lethality during fetal growth through weaning, incomplete penetrance ( J:297024 )

• percentage of embryos is normal at E11.5 and E15.5 but is decreased at E17.5 (14%), indicating that lethality may occur as early as E15.5-E17.5

• only 8.76% of homozygous pups are obtained 1 week after birth, lower than the expected 25%

growth/size/body

decreased body size ( J:297024 )

decreased body weight ( J:297024 )

reproductive system

abnormal oocyte morphology ( J:297024 )

• mice show more portions of non-surrounded nucleolus-type germinal vesicle oocytes than surrounded nucleolus-type germinal vesicle oocytes compared to wild-type mice, indicating oocyte maturation defects

• chromatin is decondensed in germinal vesicle oocytes

oligozoospermia ( J:297024 )

• loss of sperm is seen in the epididymis and testes

abnormal female meiosis ( J:297024 )

• most oocytes at the germinal vesicle stage cannot resume meiosis and mature to the MII stage with in vitro maturation

decreased mature ovarian follicle number ( J:297024 )

• ovaries possess less mature follicles, even after superovulation with gondadotropins

small ovary ( J:297024 )

small testis ( J:297024 )

infertility ( J:297024 )

• both male and female mice are infertile with mated with wild-type mice

cellular

oligozoospermia ( J:297024 )

• loss of sperm is seen in the epididymis and testes

abnormal mitosis ( J:297024 )

• percentage of embryonic stem cells in the S phase is decreased, but the percentage of G1 and G2/M phases is increased

• chromatin is decondensed in embryonic stem cells

abnormal cell differentiation ( J:297024 )

• although formation of embryoid bodies from embryonic stem cells appears normal, expression of mesoderm maker genes fail to be activated and activation of ectoderm and endoderm maker genes is irregular, indicating abnormal cell differentiation

abnormal oocyte morphology ( J:297024 )

• mice show more portions of non-surrounded nucleolus-type germinal vesicle oocytes than surrounded nucleolus-type germinal vesicle oocytes compared to wild-type mice, indicating oocyte maturation defects

• chromatin is decondensed in germinal vesicle oocytes

abnormal female meiosis ( J:297024 )

• most oocytes at the germinal vesicle stage cannot resume meiosis and mature to the MII stage with in vitro maturation

decreased cell proliferation ( J:297024 )

• embryonic stem cells derived from blastocysts grow slower during passaging in culture

maternal effect ( J:297024 )

• in IVF using homozygous mutant MII oocytes and wild-type sperm, only 50% of embryos develop to the blastocyst stage

endocrine/exocrine glands

decreased mature ovarian follicle number ( J:297024 )

• ovaries possess less mature follicles, even after superovulation with gondadotropins

small ovary ( J:297024 )

small testis ( J:297024 )