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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Riok2tm1c(KOMP)Wtsi
targeted mutation 1c, Wellcome Trust Sanger Institute
MGI:6712634
Summary 7 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Trp53tm1Tyj/Trp53tm1Tyj
Riok2tm1c(KOMP)Wtsi/Riok2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
involves: 129S2/SvPas * C57BL/6N * C57BL/10 * CBA/Ca MGI:6712734
cn2
Riok2tm1c(KOMP)Wtsi/Riok2+
Gt(ROSA)26Sortm1(cre/ERT2)Tyj/Gt(ROSA)26Sor+
involves: 129S4/SvJae * C57BL/6 * C57BL/6N MGI:6712678
cn3
Il22tm1.1Lex/Il22tm1.1Lex
Riok2tm1c(KOMP)Wtsi/Riok2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
involves: 129S5/SvEvBrd * C57BL/6N * C57BL/10 * CBA/Ca MGI:6712732
cn4
Il22ra1tm1.1Koll/Il22ra1tm1.1Koll
Riok2tm1c(KOMP)Wtsi/Riok2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
involves: C57BL/6N * C57BL/10 * CBA/Ca MGI:6712737
cn5
Riok2tm1c(KOMP)Wtsi/Riok2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
involves: C57BL/6N * C57BL/10 * CBA/Ca MGI:6712638
cn6
Riok2tm1c(KOMP)Wtsi/Riok2tm1c(KOMP)Wtsi
Commd10Tg(Vav1-icre)A2Kio/Commd10+
involves: C57BL/6N * C57BL/10 * CBA/Ca MGI:6712637
cn7
Riok2tm1c(KOMP)Wtsi/Riok2+
Tg(Cd4-cre)1Cwi/0
involves: C57BL/6 * C57BL/6N * DBA/2 MGI:6712681


Genotype
MGI:6712734
cn1
Allelic
Composition
Trp53tm1Tyj/Trp53tm1Tyj
Riok2tm1c(KOMP)Wtsi/Riok2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
Genetic
Background
involves: 129S2/SvPas * C57BL/6N * C57BL/10 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Commd10Tg(Vav1-icre)A2Kio mutation (3 available); any Commd10 mutation (24 available)
Riok2tm1c(KOMP)Wtsi mutation (0 available); any Riok2 mutation (33 available)
Trp53tm1Tyj mutation (12 available); any Trp53 mutation (239 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• the increase in IL-22 secretion seen in in vitro polarized TH22 cells in single conditional Riok2 heterozygotes is blunted




Genotype
MGI:6712678
cn2
Allelic
Composition
Riok2tm1c(KOMP)Wtsi/Riok2+
Gt(ROSA)26Sortm1(cre/ERT2)Tyj/Gt(ROSA)26Sor+
Genetic
Background
involves: 129S4/SvJae * C57BL/6 * C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(cre/ERT2)Tyj mutation (3 available); any Gt(ROSA)26Sor mutation (992 available)
Riok2tm1c(KOMP)Wtsi mutation (0 available); any Riok2 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• tamoxifen-induced mice develop anemia
• tamoxifen-induced mice exhibit reduced peripheral blood red blood cell numbers
• tamoxifen-induced mice exhibit reduced hematocrit
• tamoxifen-induced mice exhibit reduced hemoglobin




Genotype
MGI:6712732
cn3
Allelic
Composition
Il22tm1.1Lex/Il22tm1.1Lex
Riok2tm1c(KOMP)Wtsi/Riok2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
Genetic
Background
involves: 129S5/SvEvBrd * C57BL/6N * C57BL/10 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Commd10Tg(Vav1-icre)A2Kio mutation (3 available); any Commd10 mutation (24 available)
Il22tm1.1Lex mutation (2 available); any Il22 mutation (29 available)
Riok2tm1c(KOMP)Wtsi mutation (0 available); any Riok2 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• mice show an increase in RII and RIV erythroid precursors compared to IL-22-sufficient Riok2-haploinsufficent mice on day 7 after treatments with phenylhydrazine
• mice exhibit increased numbers of peripheral blood red blood cells compared to IL-22-sufficient Riok2-haploinsufficent mice on day 7 after treatments with phenylhydrazine to induce acute hemolytic stress

homeostasis/metabolism
• mice show alleviation of the stress-induced (via phenylhydrazine treatment) anemia seen in IL-22-sufficient Riok2-haploinsufficent mice




Genotype
MGI:6712737
cn4
Allelic
Composition
Il22ra1tm1.1Koll/Il22ra1tm1.1Koll
Riok2tm1c(KOMP)Wtsi/Riok2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
Genetic
Background
involves: C57BL/6N * C57BL/10 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Commd10Tg(Vav1-icre)A2Kio mutation (3 available); any Commd10 mutation (24 available)
Il22ra1tm1.1Koll mutation (1 available); any Il22ra1 mutation (31 available)
Riok2tm1c(KOMP)Wtsi mutation (0 available); any Riok2 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
N
• mice show an improvement in peripheral blood red blood cell numbers and hematocrit levels, and an increase in RIII and RIV erythroid precursors in the bone marrow compared to Il22ra1-sufficient Riok2-haploinsufficent mice




Genotype
MGI:6712638
cn5
Allelic
Composition
Riok2tm1c(KOMP)Wtsi/Riok2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
Genetic
Background
involves: C57BL/6N * C57BL/10 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Commd10Tg(Vav1-icre)A2Kio mutation (3 available); any Commd10 mutation (24 available)
Riok2tm1c(KOMP)Wtsi mutation (0 available); any Riok2 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• 8-12 week of mice exposed to acute hemolytic stress induced by phenylhydrazine treatment succumb faster to a lethal dose of phenylhydrazine than controls

hematopoietic system
• mice exhibit impaired erythropoiesis in the bone marrow
• mice over 60 weeks of age exhibit anemia
• 8-12 week of mice exposed to acute hemolytic stress (induced by nonlethal phenylhydrazine treatment) develop more severe anemia and have a delayed red blood cell recovery response compared to controls and succumb faster to a lethal dose of phenylhydrazine
• wild-type mice transplanted with mutant whole bone marrow develop anemia
• treatment of mice with a neutralizing IL-22 antibody reverses phenylhydrazine-induced anemia
• the percentage of proliferating granulocyte-macrophage progenitors in the bone marrow is increased
• anemia in young phenylhydrazine-administered mice seen on day 7 is preceded by a reduction in bone marrow RIII and RIV erythroid precursor frequency on day 6, indicating an erythroid differentiation defect
• aged mice exhibit reduced peripheral blood red blood cell numbers
• in aged mice
• mice exhibit a decreased percentage of neutrophils (neutropenia)
• aged mice show increased numbers of natural killer T cells
• mice exhibit an increased percentage of monocytes (monocytosis)
• LSK (lineage-Sca-1+Kit+) cells from the bone marrow supplemented with growth factors give rise to an increased percentage of CD11b+ myeloid cells, indicating a cell-intrinsic myeloproliferative effect
• frequency and numbers of early hematopoietic progenitors are normal in young mice, however long-term hematopoietic stem cells (LT-HSCs) are increased in the bone marrow of aged mice
• aged mice show increased numbers of splenic IL-22+CD4+T cells
• mice exhibit an increase in apoptosis of erythroid precursors
• erythroid precursors show a decrease in cell quiescence with cell cycle block at the G1 phase
• treatment of mice with a neutralizing IL-22 antibody reduces the frequency of apoptotic erythroid precursors
• TH22 cells secrete elevated concentrations of IL-22
• bone marrow cells show reduced nascent protein synthesis in vivo

homeostasis/metabolism
• the concentration of IL-22 in the serum and bone marrow fluid of aged mice is higher than in controls
• 8-12 week of mice exposed to acute hemolytic stress induced by phenylhydrazine treatment succumb faster to a lethal dose of phenylhydrazine than controls
• 8-12 week of mice exposed to acute hemolytic stress induced by nonlethal phenylhydrazine treatment develop more severe anemia and have a delayed red blood cell recovery response compared to controls
• treatment of mice with a neutralizing IL-22 antibody reverses phenylhydrazine-induced anemia

immune system
• TH22 cells secrete elevated concentrations of IL-22
• the concentration of IL-22 in the serum and bone marrow fluid of aged mice is higher than in controls
• aged mice show increased numbers of innate lymphoid cells
• mice exhibit a decreased percentage of neutrophils (neutropenia)
• aged mice show increased numbers of natural killer T cells
• mice exhibit an increased percentage of monocytes (monocytosis)
• aged mice show increased numbers of splenic IL-22+CD4+T cells
• naive T cells polarized toward the TH22 cell lineage show an increase in IL-22 secretion




Genotype
MGI:6712637
cn6
Allelic
Composition
Riok2tm1c(KOMP)Wtsi/Riok2tm1c(KOMP)Wtsi
Commd10Tg(Vav1-icre)A2Kio/Commd10+
Genetic
Background
involves: C57BL/6N * C57BL/10 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Commd10Tg(Vav1-icre)A2Kio mutation (3 available); any Commd10 mutation (24 available)
Riok2tm1c(KOMP)Wtsi mutation (0 available); any Riok2 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no homozygous mice are recovered




Genotype
MGI:6712681
cn7
Allelic
Composition
Riok2tm1c(KOMP)Wtsi/Riok2+
Tg(Cd4-cre)1Cwi/0
Genetic
Background
involves: C57BL/6 * C57BL/6N * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Riok2tm1c(KOMP)Wtsi mutation (0 available); any Riok2 mutation (33 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• mild anemia
• peripheral blood red blood cell numbers are mildly reduced
• hematocrit levels are mildly reduced
• hemoglobin levels are mildly reduced





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory