All Phenotypes Elavl2<tm1.2Ysa> MGI Mouse

• less than 20% of homozygotes survive to adulthood

postnatal lethality, incomplete penetrance ( J:282136 )

• although pups are born in Mendelian rations, >80% of homozygotes die by the weaning stage due to growth retardation

postnatal growth retardation ( J:282136 )

reproductive system phenotype ( J:282136 )

N	• adult testes exhibit no apparent morphological abnormalities

absent oocytes ( J:282136 )

• adult ovaries lack oocytes

oocyte degeneration ( J:282136 )

• oocytes are progressively lost after birth; hardly any oocytes are detected at P7

abnormal ovary morphology ( J:282136 )

• quantification of TEX14 and MKLP1 foci revealed that the number of intercellular bridges (ICBs) -- cell junctions that connect oocytes in cysts -- is nearly doubled in newborn (P1) ovaries, indicating that cyst breakdown is severely impaired

• newborn ovaries show impaired assembly of P-body-like granules; protein expression of DDX6 (a component of P-bodies and stress granules) is decreased to 34% of that in wild-type ovaries while the area of DDX6 foci is reduced to about one-fourth and expression of P-body components DCP1A and AGO2 is diminished

absent primordial ovarian follicles ( J:282136 )

• at PO, primordial follicles are hardly detectable in the ovarian medullary area

small ovary ( J:282136 )

• adult ovaries are markedly small in size

female infertility ( J:282136 )