Phenotypes associated with this allele

• Allele Symbol: Qprt^{Gt(OST337946)Lex}
• Allele Name: gene trap OST337946, Lexicon Genetics
• Allele ID: MGI:6723745
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Qprt^Gt(OST337946)Lex/Qprt^Gt(OST337946)Lex	involves: 129S5/SvEvBrd * C57BL/6J	MGI:6728100

Genotype
MGI:6728100

hm1

• Allelic Composition: Qprt^{Gt(OST337946)Lex}/Qprt^{Gt(OST337946)Lex}
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal mitochondrial morphology ( J:282516 )

• peritoneal macrophages show disrupted mitochondrial morphology

abnormal oxidative phosphorylation ( J:282516 )

• peritoneal macrophages exhibit suppressed oxidative phosphorylation

• treatment of peritoneal macrophages with NAD+ precursor beta-nicotinamide mononucleotide (NMN) normalizes oxidative phosphorylation

homeostasis/metabolism

abnormal cytokine level ( J:282516 )

• mice show increased basal plasma proinflammatory cytokines

• mice systemically stimulated with LPS show increased plasma proinflammatory cytokines

hematopoietic system

abnormal macrophage morphology ( J:282516 )

• peritoneal macrophages show disrupted mitochondrial morphology

abnormal macrophage physiology ( J:282516 )

• peritoneal macrophages exhibit decreased NAD+ abundance, suppressed oxidative phosphorylation, and increased extracellular acidification rate

• treatment of peritoneal macrophages with NAD+ precursor beta-nicotinamide mononucleotide (NMN) restores NAD+ levels and normalizes the oxidative phosphorylation and extracellular acidification rate

• peritoneal macrophages show increased mitochondrial reactive oxygen species generation and disrupted membrane potential

• macrophages show no NAD+ synthesis when administered isotope-labeled D4-KYN (kynurenine labeled with four deuteriums) or 13C-Trp

immune system

abnormal macrophage morphology ( J:282516 )

• peritoneal macrophages show disrupted mitochondrial morphology

abnormal macrophage physiology ( J:282516 )

• peritoneal macrophages exhibit decreased NAD+ abundance, suppressed oxidative phosphorylation, and increased extracellular acidification rate

• treatment of peritoneal macrophages with NAD+ precursor beta-nicotinamide mononucleotide (NMN) restores NAD+ levels and normalizes the oxidative phosphorylation and extracellular acidification rate

• peritoneal macrophages show increased mitochondrial reactive oxygen species generation and disrupted membrane potential

• macrophages show no NAD+ synthesis when administered isotope-labeled D4-KYN (kynurenine labeled with four deuteriums) or 13C-Trp

abnormal cytokine level ( J:282516 )

• mice show increased basal plasma proinflammatory cytokines

• mice systemically stimulated with LPS show increased plasma proinflammatory cytokines