Phenotypes associated with this allele

• Allele Symbol: Entpd8^tm1Ktak
• Allele Name: targeted mutation 1, Kiyoshi Takeda
• Allele ID: MGI:6771668
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Entpd8^tm1Ktak/Entpd8^tm1Ktak	C.Cg-Entpd8^tm1Ktak	MGI:7628169
cx2	Entpd8^tm1Ktak/Entpd8^tm1Ktak P2rx4^tm1Ando/P2rx4^tm1Ando	involves: 129S4/SvJae * BALB/c * C57BL/6	MGI:7628176

Genotype
MGI:7628169

hm1

• Allelic Composition: Entpd8^tm1Ktak/Entpd8^tm1Ktak
• Genetic Background: C.Cg-Entpd8^tm1Ktak

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

digestive/alimentary phenotype ( J:311564 )

N • phylum-level fecal bacterial composition is similar to wild-type controls

abnormal feces composition ( J:311564 )

• elevated ATP level in feces

• treatment with an antibiotics mixture reduces the fecal ATP level

abnormal intestine physiology ( J:311564 )

• elevated luminal ATP levels in the colon

increased susceptibility to induced colitis ( J:311564 )

• display more severe symptoms following dextran sodium sulfate (DSS)-induced colitis, including bleeding and pasty stool

• increased infiltration of IL-17+ CD4+ T cells, neutrophils, CD64- CD11b+ Ly6C-intermediate dendritic cells, and CD64+ CD11b+ macrophages in the colon

• in bone marrow chimera mice, mice receiving null cells develop more severe DSS-induced colitis

• treatment with an antibiotic mixture reduces the severity of DSS-induced colitis

• treatment with an anti-CD4 antibody fails to reduce the severity of DSS-induced colitis

• treatment with an anti-Gr-1 antibody to ablate neutrophils and monocytes reduces DSS-induced colitis severity

cellular

decreased apoptosis ( J:311564 )

• apoptosis of colonic Gr-1+ CD11b+ cells is inhibited

immune system

increased susceptibility to induced colitis ( J:311564 )

• display more severe symptoms following dextran sodium sulfate (DSS)-induced colitis, including bleeding and pasty stool

• increased infiltration of IL-17+ CD4+ T cells, neutrophils, CD64- CD11b+ Ly6C-intermediate dendritic cells, and CD64+ CD11b+ macrophages in the colon

• in bone marrow chimera mice, mice receiving null cells develop more severe DSS-induced colitis

• treatment with an antibiotic mixture reduces the severity of DSS-induced colitis

• treatment with an anti-CD4 antibody fails to reduce the severity of DSS-induced colitis

• treatment with an anti-Gr-1 antibody to ablate neutrophils and monocytes reduces DSS-induced colitis severity

Genotype
MGI:7628176

cx2

• Allelic Composition: Entpd8^tm1Ktak/Entpd8^tm1Ktak; P2rx4^tm1Ando/P2rx4^tm1Ando
• Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6

digestive/alimentary system

abnormal susceptibility to induced colitis ( J:311564 )

• severity of dextran sodium sulfate (DSS)-induced colitis is reduced compared to mutant mice wild-type for P2rx4

• reduction in the numbers of neutrophils and macrophages and partial reduction in the numbers of dendritic cells in mice with DSS-induced colitis compared to mutant mice wild-type for P2rx4

immune system

abnormal susceptibility to induced colitis ( J:311564 )

• severity of dextran sodium sulfate (DSS)-induced colitis is reduced compared to mutant mice wild-type for P2rx4

• reduction in the numbers of neutrophils and macrophages and partial reduction in the numbers of dendritic cells in mice with DSS-induced colitis compared to mutant mice wild-type for P2rx4