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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ewsr1tm1d(EUCOMM)Wtsi
targeted mutation 1d, Wellcome Trust Sanger Institute
MGI:6830504
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Ewsr1tm1c(EUCOMM)Wtsi/Ewsr1tm1d(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
involves: C57BL/6J MGI:6830505


Genotype
MGI:6830505
cn1
Allelic
Composition
Ewsr1tm1c(EUCOMM)Wtsi/Ewsr1tm1d(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ewsr1tm1c(EUCOMM)Wtsi mutation (1 available); any Ewsr1 mutation (93 available)
Ewsr1tm1d(EUCOMM)Wtsi mutation (0 available); any Ewsr1 mutation (93 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• In spermatocytes the number of double stranded breaks, indicated by DMC1 foci, is normal in early leptotene and zygotene but double stranded repair is delayed, PRDM-dependent hotspots differ somewhat from controls, and there are increased unsynapsed chromosomes
• meiosis is blocked at pachynema/diplonema, with normal proportions of leptotene and zygotene spermatocytes, no spermatocytes are found in seminiferous tubules beyond prophase 1, and there is increased apoptotic germ cells in testes
• spermatocytes show decreased PRDM9-dependent H3K4 and H3K36 trimethylation: decreased H3K4me3 in leptonema and zygonema and slightly decreased H3K36me3 in leptonema and zygonema but not diplonema; H3K4me3 ChIP-seq from spermatocytes identified fewer than normal peaks, with approximately 38.7% of hotspot peaks missing, in particular those with low H3K4 trimethylation in controls; reduced levels of H3K36 trimethylation, particularly in intergenic region hotspots

reproductive system
• In spermatocytes the number of double stranded breaks, indicated by DMC1 foci, is normal in early leptotene and zygotene but double stranded repair is delayed, PRDM-dependent hotspots differ somewhat from controls, and there are increased unsynapsed chromosomes
• spermatocytes show decreased PRDM9-dependent H3K4 and H3K36 trimethylation: decreased H3K4me3 in leptonema and zygonema and slightly decreased H3K36me3 in leptonema and zygonema but not diplonema; H3K4me3 ChIP-seq from spermatocytes identified fewer than normal peaks, with approximately 38.7% of hotspot peaks missing, in particular those with low H3K4 trimethylation in controls; reduced levels of H3K36 trimethylation, particularly in intergenic region hotspots
• decreased testes weight in adults but not at 14 or 18 days of age
• meiosis is blocked at pachynema/diplonema, with normal proportions of leptotene and zygotene spermatocytes, no spermatocytes are found in seminiferous tubules beyond prophase 1, and there is increased apoptotic germ cells in testes

endocrine/exocrine glands
• decreased testes weight in adults but not at 14 or 18 days of age





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory