Analysis Tools
reproductive system
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• about 8% of cauda epididymis spermatozoa show disorganized microtubule structures in axonemes
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• about 85% of cauda epididymis spermatozoa show impaired radial spoke structures
• sperm flagella show one or two radial spokes per 96 nm repeat with one or two absent or truncated radial spokes compared to wild-type that show radial spoke 1 (RS1), RS2, and RS3 arranged in 96 nm repeats
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• spermatozoa exhibit reduced progressive motility
• although progressive motility is impaired, the flagella of spermatozoa do beat, but across a more limited range
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• reduction in motile spermatozoa and spermatozoa exhibiting progressive motility
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• males are unable to sire any offspring despite successful copulation with wild-type females, indicating male infertility
• however, no differences in testis appearance or weight or in spermatogenesis, with both the cauda and caput regions of the epididymal ducts filled with spermatozoa
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• in an in vivo fertilization assay, no fertilized eggs are found in wild-type females after mating with homozygous males
• in vitro fertilization assays show that spermatozoa are unable to fertilize cumulus-intact or cumulus-free zona pellucida-intact oocytes
• however, spermatozoa are able to fuse with oocytes following zona pellucida removal
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cellular
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• about 8% of cauda epididymis spermatozoa show disorganized microtubule structures in axonemes
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• about 85% of cauda epididymis spermatozoa show impaired radial spoke structures
• sperm flagella show one or two radial spokes per 96 nm repeat with one or two absent or truncated radial spokes compared to wild-type that show radial spoke 1 (RS1), RS2, and RS3 arranged in 96 nm repeats
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• spermatozoa exhibit reduced progressive motility
• although progressive motility is impaired, the flagella of spermatozoa do beat, but across a more limited range
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• reduction in motile spermatozoa and spermatozoa exhibiting progressive motility
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respiratory system
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N |
• lung sections show no obvious alterations in respiratory cilia, cilia length is unaltered in tracheal epithelial cells, and tracheal ciliary beating is comparable to wild-type
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