Phenotypes associated with this allele

• Allele Symbol: Or12d17^em1Cya
• Allele Name: endonuclease-mediated mutation 1, Cyagen Biosciences
• Allele ID: MGI:6887709
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Or12d17^em1Cya/Or12d17^em1Cya	C57BL/6N-Or12d17^em1Cya	MGI:7664083
cx2	Ins2^Akita/Ins2^+ Or12d17^em1Cya/Or12d17^em1Cya	C57BL/6-Ins2^Akita Or12d17^em1Cya	MGI:7664100

Genotype
MGI:7664083

hm1

• Allelic Composition: Or12d17^em1Cya/Or12d17^em1Cya
• Genetic Background: C57BL/6N-Or12d17^em1Cya

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased susceptibility to diet-induced obesity ( J:321591 )

• mice fed a high-fat diet (HFD) show less weight gain that wild-type mice at 13 weeks of age

behavior/neurological

behavior/neurological phenotype ( J:321591 )

N • HFD-fed mice show no differences in locomotor activity or food intake

homeostasis/metabolism

decreased circulating leptin level ( J:321591 )

• fasting leptin levels are lower in HFD-fed mice

increased energy expenditure ( J:321591 )

• HFD-fed mice show increased energy expenditure

• however, mice fed regular chow show normal energy expendiature

decreased susceptibility to diet-induced obesity ( J:321591 )

• mice fed a high-fat diet (HFD) show less weight gain that wild-type mice at 13 weeks of age

increased respiratory quotient ( J:321591 )

• HFD-fed mice show increased respiratory quotient values at 12 weeks of age

• however, mice fed regular chow show normal respiratory quotient

abnormal glucose homeostasis ( J:321591 )

• mice fed a high-fat diet show improved glucose homeostasis compared to wild-type mice

increased insulin secretion ( J:321591 )

• high-glucose-induced insulin secretion is increased under both normal and HFD-induced conditions in pancreatic islets

decreased circulating glucose level ( J:321591 )

• plasma glucose level is decreased after refeeding in HFD-fed mice

decreased fasting circulating glucose level ( J:321591 )

• fasting glucose levels are lower in HFD-fed mice

decreased circulating insulin level ( J:321591 )

• fasting insulin levels are lower in HFD-fed mice

increased circulating insulin level ( J:321591 )

• plasma insulin level is increased after refeeding in HFD-fed mice

improved glucose tolerance ( J:321591 )

• HFD-fed mice exhibit improved glucose metabolism in the glucose tolerance test as early as 8 weeks

• change in glucose tolerance occurs before the improvement in insulin resistance in HFD-fed mice

• however, mice fed a regular chow diet show normal glucose tolerance

increased insulin sensitivity ( J:321591 )

• HFD-fed mice show decreased insulin resistance at 13 weeks of age, but not at earlier ages

adipose tissue

decreased white adipose tissue amount ( J:321591 )

• HFD-fed mice show lower white adipose tissue weight than wild-type mice at 12 weeks of age

decreased white fat cell size ( J:321591 )

• HFD-fed mice show a decrease in the epididymal adipocyte size

endocrine/exocrine glands

increased insulin secretion ( J:321591 )

• high-glucose-induced insulin secretion is increased under both normal and HFD-induced conditions in pancreatic islets

liver/biliary system

decreased liver weight ( J:321591 )

• HFD-fed mice show lower liver weights than wild-type mice at 12 weeks of age

decreased susceptibility to diet-induced hepatic steatosis ( J:321591 )

• HFD-fed mice show reduced liver steatosis compared to wild-type mice

Genotype
MGI:7664100

cx2

• Allelic Composition: Ins2^Akita/Ins2⁺; Or12d17^em1Cya/Or12d17^em1Cya
• Genetic Background: C57BL/6-Ins2^Akita Or12d17^em1Cya

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:321591 )

N • islets show increased expression of pancreatic beta cell markers in islets than that seen in Ins2^Akita mice, indicating rescue of the reduced pancreatic beta cells in islets

abnormal pancreatic islet morphology ( J:321591 )

• mice show partial rescue of the decreased insulin content in islets seen in Ins2^Akita mice but insulin content is still lower than in wild-type mice

• mice show partial rescue of the increased CD11c+ macrophages in islets of Ins2^Akita mice

growth/size/body

growth/size/body region phenotype ( J:321591 )

N • mice show rescue of the decreased body weight seen in heterozygous Ins2^Akita mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:321591 )

N • mice show reversal of the increased energy expenditure and reduced respiratory quotient seen in the dark photo-period of heterozygous Ins2^Akita mice

decreased circulating insulin level ( J:321591 )

• mice show improved glucose-induced plasma insulin levels at 8 weeks of age compared to heterozygous Ins2^Akita mice but not fully to wild-type levels

impaired glucose tolerance ( J:321591 )

• mice show markedly improved glucose tolerance at 8 weeks of age compared to heterozygous Ins2^Akita mice but not fully to wild-type levels