Phenotypes associated with this allele

• Allele Symbol: Agmo^{tm2a(EUCOMM)Wtsi}
• Allele Name: targeted mutation 2a, Wellcome Trust Sanger Institute
• Allele ID: MGI:7256325
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Agmo^tm2a(EUCOMM)Wtsi/Agmo^tm2a(EUCOMM)Wtsi	involves: C57BL/6J * C57BL/6N	MGI:7703607
hm2	Agmo^tm2a(EUCOMM)Wtsi/Agmo^tm2a(EUCOMM)Wtsi	involves: C57BL/6N	MGI:7256335

Genotype
MGI:7703607

hm1

• Allelic Composition: Agmo^{tm2a(EUCOMM)Wtsi}/Agmo^{tm2a(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6J * C57BL/6N
• Cell Lines: EPD0354_2_F05

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal ether lipid level ( J:333103 )

♂ • mice show a modest accumulation of plasmanyl and plasmenyl lipid species in brain (cerebrum and cerebellum), liver, and subcutaneous and visceral white adipose tissue (sWAT and vWAT, respectively)

♂ • liver shows the most pronounced but still mild changes, with a moderate elevation of both plasmanyl and plasmenyl lipid species in all ether lipid subclasses except for plasmanyl phosphatidylcholine (PC[O]), plasmanyl phosphatidylethanolamine (PE[O]) and plasmenyl phosphatidylethanolamine (PE[P]); in contrast, only certain ether lipid subclasses are affected in the other tissues

♂ • sWAT and vWAT shows an accumulation of neutral ether lipids, i.e. plasmanyl/plasmenyl triacylglycerol (TG[O + P])

♂ • both brain regions (cerebrum and cerebellum) show significantly higher levels of plasmanyl lysophosphatidylcholine (LPC[O]), plasmenyl lysophosphatidylcholine (LPC[P]) and PC[O], whereas plasmanyl/plasmenyl phosphatidylethanolamine (PE[O/P]) is unaffected

♂ • moderate accumulation of AGMO substrates plasmanyl monoacylglycer (MG[O]) and LPC[O] as well as LPC[P] is seen in cerebrum

♂ • sWAT only shows enhanced levels of AGMO substrates plasmanyl lysophosphatidylethanolamine (LPE[O]), LPC[O], and MG[O]

growth/size/body

growth/size/body region phenotype ( J:333103 )

♂N • unchallenged mice do not exhibit an obvious phenotype under SPF conditions

Genotype
MGI:7256335

hm2

• Allelic Composition: Agmo^{tm2a(EUCOMM)Wtsi}/Agmo^{tm2a(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6N
• Cell Lines: EPD0354_2_F05

mortality/aging

mortality/aging ( J:308367 )

N • qPCR confirmed mice were born in Mendelian ratios

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:308367 )

N • female and male mice exhibit normal triglyceride while only female mice exhibit normal cholesterol levels

decreased circulating cholesterol level ( J:308367 )

♂ • in male, but not female, mice

abnormal enzyme/coenzyme activity ( J:308367 )

• absent alkylglycerol monooxygenase activity

adipose tissue

adipose tissue phenotype ( J:308367 )

N • mice exhibit normal body fat volume