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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Mirc33em1Smoc
endonuclease-mediated mutation 1, Shanghai Model Organisms Center
MGI:7264934
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Mirc33em1Smoc/Mirc33em1Smoc Not Specified MGI:7276138


Genotype
MGI:7276138
hm1
Allelic
Composition
Mirc33em1Smoc/Mirc33em1Smoc
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• at the onset of puberty, females are significantly lighter than wild-type controls

reproductive system
N
• at 8 weeks of age, male mice exhibit normal testis and seminal vesicle weight to body weight ratio
• although early ovarian follicles develop normally, the number of corpora lutea per ovary is significantly lower than in wild-type females
• however, corpora lutea formation is rescued by exogenous gonadotropin injection
• wet ovary weight to body weight ratio is significantly decreased at 8 weeks of age
• expression of Cyp11a1, which is essential to the level of sex hormones, is significantly decreased in the ovary
• however, ovarian expression of Cyp19a1 and Cyp17a1, encoding enzymes involved in estradiol and testosterone synthesis, is normal
• uterus weight to body weight ratio is significantly decreased at 8 weeks of age
• vaginal opening occurs 5 days later (P28) than in wild-type females (P23)
• during natural ovulation, only 2 oocytes were collected from the oviducts of 20 females with a visible plug
• however, ovulation is rescued by exogenous gonadotropin injection
• females display irregular estrous cycles
• females spend significantly more time in diestrus
• females spend significantly less time in estrus
• females spend significantly less time in metestrus
• 23 of the 25 virgin 8-week-old females tested failed to conceive within 1 month of mating and were defined as sterile
• 2 of the 25 virgin 8-week-old females tested gave birth to 2 pups each but did not become subsequently pregnant
• females produce a mean litter size of 2 pups/litter versus 7.6 pups/litter in wild-type females
• 66.7% of 8-week-old males were still fertile
• during natural ovulation, only 2 oocytes were collected from the oviducts of 20 females with a visible plug and no 2-cell embryos were found the following day
• however, the number of 2-cell embryos obtained is restored to nearly wild-type levels after exogenous gonadotropin injection

nervous system
• gonadotropin releasing hormone receptor (GnRHR)-immunoreactivity is significantly increased in the pituitary
• females show impaired pituitary hormone secretion, especially LH released

homeostasis/metabolism
N
• female mice show normal serum follicle-stimulating hormone (FSH), testosterone and estradiol (E2) levels
• females show a significant reduction in serum luteinizing hormone (LH) levels
• despite normal GnRH responsiveness in the pituitary, serum LH levels remain markedly below wild-type levels after i.p. injection of exogenous GnRH
• after ovariectomy, females fail to show a significant rise in serum LH levels unlike ovariectomized wild-type females, indicating a block in LH secretion
• however, rise in serum FSH levels is relatively normal after ovariectomy
• females show a significant reduction in serum progesterone (P4) levels
• expression of Cyp11a1, which is essential to the level of sex hormones, is significantly decreased in the ovary
• however, ovarian expression of Cyp19a1 and Cyp17a1, encoding enzymes involved in estradiol and testosterone synthesis, is normal
• LH protein levels and immunoreactivity are significantly increased in the pituitary

behavior/neurological
• females show a significant lower mating frequency than wild-type females

endocrine/exocrine glands
• although early ovarian follicles develop normally, the number of corpora lutea per ovary is significantly lower than in wild-type females
• however, corpora lutea formation is rescued by exogenous gonadotropin injection
• wet ovary weight to body weight ratio is significantly decreased at 8 weeks of age
• gonadotropin releasing hormone receptor (GnRHR)-immunoreactivity is significantly increased in the pituitary
• females show impaired pituitary hormone secretion, especially LH released
• expression of Cyp11a1, which is essential to the level of sex hormones, is significantly decreased in the ovary
• however, ovarian expression of Cyp19a1 and Cyp17a1, encoding enzymes involved in estradiol and testosterone synthesis, is normal

cellular
• females show abnormal expression of several proteins involved in vesicular transport in the pituitary
• females show abnormal expression of several proteins involved in exocytosis in the pituitary





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory