Analysis Tools
homeostasis/metabolism
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• mice show no overt bleeding and have normal plasma and platelet von Willebrand factor levels and normal bleeding time and wound blood loss after tail transection (up to 900 s) relative to wild-type controls
• platelets show normal low shear light transmission aggregometry (LTA) responses to thrombin, TRAP, collagen, and ADP; normal whole blood aggregometry (WBA) responses to collagen; and normal shear-induced platelet aggregation (SIPA) over a range of shear rates
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• in high shear flow assays using whole blood, platelets show decreased adhesion to Horm collagen and fail to form the large adherent aggregates observed with wild-type platelets; real-time analysis indicates less initial adhesion and slower growth of platelet aggregates on collagen
• under static conditions, collagen-related peptide (CRP)-activated platelets show impaired platelet adhesion to Horm collagen but normal adhesion to the triple-helical collagen peptide GFOGER (a high-affinity alpha2beta1 ligand)
• under low shear flow conditions, washed platelets show impaired adhesion to immobilized Horm collagen
• under high shear flow conditions, both whole blood and washed platelets show normal adhesion to recombinant von Willebrand factor (rVwf) albeit with a reduction in the size of captured platelet aggregates
• in low shear flow assays using whole blood, activated platelets show a minor decrease in adhesion to immobilized murine fibrinogen with a reduction in the size of captured platelet aggregates, but show normal adhesion to fibrin and fibronectin
• under high shear flow conditions, washed CRP-activated platelets show impaired platelet adhesion to GPAGPOGPX motifs in fibrillar collagens with smaller platelet aggregates captured on surfaces coated with GFOGER plus GPP only
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• thrombin-stimulated platelets show a minor increase in the expression of activated alphaIIbbeta3 (glycoprotein IIb/IIIa, aka CD41/CD61) on their surface relative to wild-type controls
• however, thrombin-induced P-selectin (CD62P) expression on the platelet surface is normal
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• in the FeCl3-induced mesenteric vessel injury model, mice have a significantly lower number of platelets localized to the vessel wall 3-5 min post-injury and show delayed appearance of the first large thrombus (greater or equal to 20 um)
• 2 of 9 mice fail to form a large thrombus; when large thrombi are formed, small platelet aggregates dissociate rapidly
• 3 of 8 mice fail to form an occlusive thrombus by 40 min, whereas all (11 of 11) wild-type controls form an occlusive thrombus by 10 to 35 min after injury
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hematopoietic system
| N |
• mice show normal platelet, erythrocyte and leukocyte counts relative to wild-type controls
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• in high shear flow assays using whole blood, platelets show decreased adhesion to Horm collagen and fail to form the large adherent aggregates observed with wild-type platelets; real-time analysis indicates less initial adhesion and slower growth of platelet aggregates on collagen
• under static conditions, collagen-related peptide (CRP)-activated platelets show impaired platelet adhesion to Horm collagen but normal adhesion to the triple-helical collagen peptide GFOGER (a high-affinity alpha2beta1 ligand)
• under low shear flow conditions, washed platelets show impaired adhesion to immobilized Horm collagen
• under high shear flow conditions, both whole blood and washed platelets show normal adhesion to recombinant von Willebrand factor (rVwf) albeit with a reduction in the size of captured platelet aggregates
• in low shear flow assays using whole blood, activated platelets show a minor decrease in adhesion to immobilized murine fibrinogen with a reduction in the size of captured platelet aggregates, but show normal adhesion to fibrin and fibronectin
• under high shear flow conditions, washed CRP-activated platelets show impaired platelet adhesion to GPAGPOGPX motifs in fibrillar collagens with smaller platelet aggregates captured on surfaces coated with GFOGER plus GPP only
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• thrombin-stimulated platelets show a minor increase in the expression of activated alphaIIbbeta3 (glycoprotein IIb/IIIa, aka CD41/CD61) on their surface relative to wild-type controls
• however, thrombin-induced P-selectin (CD62P) expression on the platelet surface is normal
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