About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tesminem2Qsh
endonuclease-mediated mutation 2, Qinghua Shi
MGI:7276187
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Tesminem2Qsh/Tesminem2Qsh involves: C57BL/6J MGI:7327255


Genotype
MGI:7327255
hm1
Allelic
Composition
Tesminem2Qsh/Tesminem2Qsh
Genetic
Background
involves: C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tesminem2Qsh mutation (0 available); any Tesmin mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• at 10 weeks of age, no sperm are detected in the cauda epididymis, as observed in homozygous Tesminem1Qsh males
• immunostaining for SYCP3 and SYCP1 (synapsis makers between homologous chromosomes) on spermatocyte spreads showed complete absence of diplotene spermatocytes among meiotic prophase I cells
• ratios of leptotene and zygotene spermatocytes are significantly increased (due to lack of diplotene cells)
• however, ratio of pachytene spermatocytes is not significantly altered, indicating loss of pachytene spermatocytes
• TUNEL staining showed that the % of seminiferous tubules with TUNEL+ cells and number of TUNEL+ cells (spermatocytes) per tubule is significantly higher than that in wild-type testes but comparable to that in homozygous Tesminem1Qsh testes
• at 10 weeks of age, testis size is significantly smaller than in wild-type controls but comparable to that in homozygous Tesminem1Qsh males
• at 10 weeks of age, testis/body weight ratio is reduced to only 2.16 versus ~8 in wild-type controls but is comparable to that observed in homozygous Tesminem1Qsh males

cellular
• at 10 weeks of age, no sperm are detected in the cauda epididymis, as observed in homozygous Tesminem1Qsh males
• immunostaining for SYCP3 and SYCP1 (synapsis makers between homologous chromosomes) on spermatocyte spreads showed complete absence of diplotene spermatocytes among meiotic prophase I cells
• ratios of leptotene and zygotene spermatocytes are significantly increased (due to lack of diplotene cells)
• however, ratio of pachytene spermatocytes is not significantly altered, indicating loss of pachytene spermatocytes
• TUNEL staining showed that the % of seminiferous tubules with TUNEL+ cells and number of TUNEL+ cells (spermatocytes) per tubule is significantly higher than that in wild-type testes but comparable to that in homozygous Tesminem1Qsh testes

endocrine/exocrine glands
• at 10 weeks of age, testis size is significantly smaller than in wild-type controls but comparable to that in homozygous Tesminem1Qsh males
• at 10 weeks of age, testis/body weight ratio is reduced to only 2.16 versus ~8 in wild-type controls but is comparable to that observed in homozygous Tesminem1Qsh males





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
06/12/2024
MGI 6.13
The Jackson Laboratory