All Phenotypes Tm9sf5<em1Dzha> MGI Mouse

abnormal oocyte morphology ( J:320690 )

• fully-grown oocytes exhibit significantly more gamma-H2AX signal dots than wild-type oocytes

• oocyte maturation (MII rate) and the number of ovulated oocytes is significantly reduced, indicating impaired oocyte quality

• intracellular ATP levels and mitochondrial membrane potential are significantly decreased, whereas mitochondria aggregation, reactive oxygen species (ROS) production and aneuploidy are significantly increased

• ovulated oocytes show significantly more variable expression of NICD2 and p-AKT^S473 than wild-type oocytes

abnormal ovarian follicle number ( J:320690 )

• at 2 months of age, numbers of total follicles, primordial follicles, secondary follicles and antral follicles per ovary are significantly lower than in wild-type females

decreased primordial ovarian follicle number ( J:320690 )

decreased secondary ovarian follicle number ( J:320690 )

abnormal tertiary ovarian follicle morphology ( J:320690 )

• antral follicles contain significantly more gamma-H2AX signal dots than wild-type antral follicles

decreased tertiary ovarian follicle number ( J:320690 )

decreased ovulation rate ( J:320690 )

• number of ovulated oocytes is significantly lower than in wild-type females

reduced female fertility ( J:320690 )

• fertility assays from 8 to 44 weeks showed a significant reduction in the number of cumulative pups per female and in the number of cumulative pups per month relative to wild-type females

abnormal oocyte morphology ( J:320690 )

• fully-grown oocytes exhibit significantly more gamma-H2AX signal dots than wild-type oocytes

• oocyte maturation (MII rate) and the number of ovulated oocytes is significantly reduced, indicating impaired oocyte quality

• intracellular ATP levels and mitochondrial membrane potential are significantly decreased, whereas mitochondria aggregation, reactive oxygen species (ROS) production and aneuploidy are significantly increased

• ovulated oocytes show significantly more variable expression of NICD2 and p-AKT^S473 than wild-type oocytes

aneuploidy ( J:320690 )

• MII oocytes show a significantly higher aneuploidy rate (%) than wild-type oocytes

enhanced autophagy ( J:320690 )

• oocytes exhibit significantly increased lysosome intensity, suggesting an abnormally high autophagy level

abnormal mitochondrial physiology ( J:320690 )

• oocytes exhibit significantly decreased intracellular ATP levels, increased mitochondria aggregation, decreased mitochondrial membrane potential and increased ROS level, suggesting mitochondrial dysfunction

oxidative stress ( J:320690 )

• oocytes show a significant increase in reactive oxygen species (ROS) level along with a significant decrease in SOD2 (superoxide dismutase 2, mitochondrial) protein level

maternal effect ( J:320690 )

• at 10 dpc, some implanted embryos within the uterus are significantly smaller than in wild-type controls

• at 14 or 18 dpc, significantly more embryos dissected from the uterus are developmentally abnormal (tiny and absorbed)

• at 21 dpc, 4 of 6 of embryos show significantly lower NICD2 and p-AKT^S473 levels, whereas all wild-type embryos have higher and similar NICD2 and p-AKT^S473 levels

• analysis of in vitro development of in vivo fertilized oocytes showed that the rate of blastula formation is significantly lower than that of wild-type fertilized oocytes (35.5% versus 68.1%, respectively)