Analysis Tools
immune system
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• mice show no significant differences in the distribution of B cells, macrophages, monocytes, dendritic cells, and granulocytes relative to wild-type controls
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• mice exhibit enhanced alpha4 integrin-mediated T cell migration and homing to the gut
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• in a T cell-transfer model of chronic colitis, Rag1 null mice reconstituted with naive T cells (CD4+CD45RBhighCD25low) exhibit higher colitis clinical scores, increased infiltration of mononuclear cells in colonic lamina propria, more severe destruction of the epithelial barrier, and significantly more alpha4+ CD4+ T cells in the colon than control mice receiving wild-type naive T cells
• however, in vitro, naive T cells show normal proliferation and activation capacities relative to wild-type naive T cells
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• flow cytometry analysis of T cell distribution showed significantly more T cells in the small intestine, colon, and Peyers patches than in wild-type controls
• however, T cell distribution in the bone marrow, thymus, spleen, and peripheral and mesenteric lymph nodes is normal
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• CD4+ T cells show increased talin binding to alpha4 integrins and enhanced binding to soluble VCAM-1 and MAdCAM-1 (mucosal addressin cell adhesion molecule-1)
• however, splenic CD4+ T cells show normal expression levels of alpha4 integrin
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hematopoietic system
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• mice exhibit enhanced alpha4 integrin-mediated T cell migration and homing to the gut
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• flow cytometry analysis of T cell distribution showed significantly more T cells in the small intestine, colon, and Peyers patches than in wild-type controls
• however, T cell distribution in the bone marrow, thymus, spleen, and peripheral and mesenteric lymph nodes is normal
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• CD4+ T cells show increased talin binding to alpha4 integrins and enhanced binding to soluble VCAM-1 and MAdCAM-1 (mucosal addressin cell adhesion molecule-1)
• however, splenic CD4+ T cells show normal expression levels of alpha4 integrin
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cellular
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• mice exhibit enhanced alpha4 integrin-mediated T cell migration and homing to the gut
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• in a single-cell random migration assay, CD4+ T cells show enhanced migration on immobilized VCAM-1 and MAdCAM-1 (mucosal addressin cell adhesion molecule-1) substrates, with a higher average velocity than wild-type T cells
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digestive/alimentary system
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• in a T cell-transfer model of chronic colitis, Rag1 null mice reconstituted with naive T cells (CD4+CD45RBhighCD25low) exhibit higher colitis clinical scores, increased infiltration of mononuclear cells in colonic lamina propria, more severe destruction of the epithelial barrier, and significantly more alpha4+ CD4+ T cells in the colon than control mice receiving wild-type naive T cells
• however, in vitro, naive T cells show normal proliferation and activation capacities relative to wild-type naive T cells
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