All Phenotypes Rad51<tm1Csha> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rad51^tm1Csha/Rad51^tm1Csha Ndor1^{Tg(UBC-cre/ERT2)1Ejb}/Ndor1⁺	involves: 129S/Sv * C57BL/6J	MGI:7311703
cn2	Rad51^tm1Csha/Rad51^tm1Csha Tg(Ddx4-cre)1Dcas/0	involves: C57BL/6J * FVB	MGI:7311694

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased male germ cell number ( J:324159 )

• at P70, no PLZF+ spermatogonial stem cells (SSCs) are detected in the seminiferous tubules of tamoxifen-treated mice

• at P14, no pachytene spermatocytes are detected in the tubules of tamoxifen-treated mice

increased male germ cell apoptosis ( J:324159 )

• at P14 and P70, the number of TUNEL+ apoptotic spermatocytes in the seminiferous tubules of tamoxifen-treated mice is significantly higher than in control males

small testis ( J:324159 )

• at P70, tamoxifen-treated mice show a significantly smaller testis size than control males

decreased testis weight ( J:324159 )

• at P70, testis weight in tamoxifen-treated mice is 80% of that in control males

abnormal spermatogenesis ( J:324159 )

• tamoxifen-treated males exhibit early spermatogenic cells loss and apoptosis

abnormal spermatocyte morphology ( J:324159 )

• at P14, no pachytene spermatocytes are detected in the seminiferous tubules of tamoxifen-treated mice

• at P70, chromosome spreads from tamoxifen-treated testes show a significant decrease in leptotene, zygotene and pachytene spermatocytes with a concomitant increase in diplotene and metaphase I cells relative to control testes

• at P70, % of pachytene spermatocytes is significantly lower than in control testes (12.57% versus 41.37%) whereas % of diplotene spermatocytes is significantly increased (82.74% versus 48.83%)

abnormal male meiosis ( J:324159 )

• at P14, no meiotic cells are found in the testes of tamoxifen-treated mice; only mitotic cells are observed

• no SYCP3+ or gammaH2AX+ cells are observed in the seminiferous tubules of tamoxifen-treated mice at P14

• at P70, chromosome spreads from tamoxifen-treated testes show meiosis defects, including a significant reduction in zygotene and pachytene spermatocytes, defective double-strand DNA repair, and a significant decrease in MLH1 foci in pachytene spermatocytes indicating reduced crossover formation

cellular

abnormal spermatocyte morphology ( J:324159 )

• at P14, no pachytene spermatocytes are detected in the seminiferous tubules of tamoxifen-treated mice

• at P70, % of pachytene spermatocytes is significantly lower than in control testes (12.57% versus 41.37%) whereas % of diplotene spermatocytes is significantly increased (82.74% versus 48.83%)

decreased male germ cell number ( J:324159 )

• at P70, no PLZF+ spermatogonial stem cells (SSCs) are detected in the seminiferous tubules of tamoxifen-treated mice

• at P14, no pachytene spermatocytes are detected in the tubules of tamoxifen-treated mice

abnormal male meiosis ( J:324159 )

• at P14, no meiotic cells are found in the testes of tamoxifen-treated mice; only mitotic cells are observed

• no SYCP3+ or gammaH2AX+ cells are observed in the seminiferous tubules of tamoxifen-treated mice at P14

increased male germ cell apoptosis ( J:324159 )

• at P14 and P70, the number of TUNEL+ apoptotic spermatocytes in the seminiferous tubules of tamoxifen-treated mice is significantly higher than in control males

abnormal double-strand DNA break repair ( J:324159 )

• at P70, chromosome spreads from tamoxifen-treated testes show abnormal accumulation of gammaH2AX foci on the axes of autosomal chromosomes as well as a significant increase of DMC1 foci in pachytene spermatocytes, indicating an increased incidence of unrepaired DNA breaks

homeostasis/metabolism

abnormal double-strand DNA break repair ( J:324159 )

endocrine/exocrine glands

small testis ( J:324159 )

• at P70, tamoxifen-treated mice show a significantly smaller testis size than control males

decreased testis weight ( J:324159 )

• at P70, testis weight in tamoxifen-treated mice is 80% of that in control males

Allelic Composition	Rad51^tm1Csha/Rad51^tm1Csha Tg(Ddx4-cre)1Dcas/0
Genetic Background	involves: C57BL/6J * FVB

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad51^tm1Csha mutation (0 available); any Rad51 mutation (31 available)
Tg(Ddx4-cre)1Dcas mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal spermatogonia morphology ( J:324159 )

• at P8, TEM showed complete loss of spermatogonia in seminiferous tubules

decreased male germ cell number ( J:324159 )

• at P12 and P60, Hematoxylin staining of seminiferous tubules showed complete germ cell loss

• at P10, no PLZF+ early spermatogonial stem cells (SSCs) are detected in seminiferous tubules

• however, GCNA+ male germ cells at still present at E18.5

abnormal seminiferous tubule morphology ( J:324159 )

• at P8, testes show severe depletion of germ cells in the seminiferous tubules; only SOX9+ Sertoli cells are detected at P8

increased Sertoli cell number ( J:324159 )

• at P19 and P40, the number of SOX9+ Sertoli cells per tubule is significantly higher than in control testes

small testis ( J:324159 )

• at P40, testis size is significantly smaller than in control males

decreased testis weight ( J:324159 )

• from P10 to P40, testis weight is significantly lower than in control males

• however, testis weight is normal at P8

abnormal spermatogenesis ( J:324159 )

• at P12, seminiferous tubules display complete loss of spermatogonia and a Sertoli cell-only phenotype

• immunofluorescence staining of the Sertoli cell marker SOX9 showed that tubules only contain Sertoli cells at P8

abnormal spermatocyte morphology ( J:324159 )

• at P12, seminiferous tubules show complete loss of leptotene and zygotene spermatocytes

abnormal male meiosis ( J:324159 )

• immunofluorescence staining of meiotic markers showed no SYCP3+ or gammaH2AX+ cells in seminiferous tubules at P8

male infertility ( J:324159 )

• adult male mice mated with wild-type fertile females for at least 6 months produce no pups

cellular

abnormal spermatocyte morphology ( J:324159 )

• at P12, seminiferous tubules show complete loss of leptotene and zygotene spermatocytes

abnormal spermatogonia morphology ( J:324159 )

• at P8, TEM showed complete loss of spermatogonia in seminiferous tubules

decreased male germ cell number ( J:324159 )

• at P12 and P60, Hematoxylin staining of seminiferous tubules showed complete germ cell loss

• at P10, no PLZF+ early spermatogonial stem cells (SSCs) are detected in seminiferous tubules

• however, GCNA+ male germ cells at still present at E18.5

abnormal male meiosis ( J:324159 )

• immunofluorescence staining of meiotic markers showed no SYCP3+ or gammaH2AX+ cells in seminiferous tubules at P8

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:324159 )

• at P8, testes show severe depletion of germ cells in the seminiferous tubules; only SOX9+ Sertoli cells are detected at P8

increased Sertoli cell number ( J:324159 )

• at P19 and P40, the number of SOX9+ Sertoli cells per tubule is significantly higher than in control testes

small testis ( J:324159 )

• at P40, testis size is significantly smaller than in control males

decreased testis weight ( J:324159 )

• from P10 to P40, testis weight is significantly lower than in control males

• however, testis weight is normal at P8

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