Phenotypes associated with this allele

• Allele Symbol: Lrrc32^tm1Zhli
• Allele Name: targeted mutation 1, Zihai Li
• Allele ID: MGI:7314986
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lrrc32^tm1Zhli/Lrrc32^tm1Zhli	Not Specified	MGI:7341658

Genotype
MGI:7341658

hm1

• Allelic Composition: Lrrc32^tm1Zhli/Lrrc32^tm1Zhli
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:248781 )

• all mice die within 24 h after birth with no apparent defects in major organs

behavior/neurological

absent gastric milk in neonates ( J:248781 )

• no milk is visible in the stomach of dead pus

craniofacial

abnormal secondary palate development ( J:248781 )

• TUNEL assays showed a significant decrease in the number of apoptotic cells in the rostral and caudal regions of the medial edge epithelium (MEE) of the palatal shelves at E14.5 and E15.5

• immunohistochemistry showed a significant reduction of SMAD2/3 phosphorylation in the rostral and caudal regions of the MEE at E14.5, indicating a defect in the TGFbeta signaling pathway

persistence of medial edge epithelium during palatal shelf fusion ( J:248781 )

• although formation and elevation of the palatal shelves is observed in the rostral region, no midline epithelial seam is seen in the caudal region at E14.5 and palatal fusion is incomplete from E15.5 to E16.5

• a significant reduction of apoptosis is detected at the MEE seam at E14.5

• however, palatal edge epithelial cell proliferation is normal

cleft palate ( J:248781 )

• all mice show a cleft palate phenotype

growth/size/body

abnormal secondary palate development ( J:248781 )

• TUNEL assays showed a significant decrease in the number of apoptotic cells in the rostral and caudal regions of the medial edge epithelium (MEE) of the palatal shelves at E14.5 and E15.5

• immunohistochemistry showed a significant reduction of SMAD2/3 phosphorylation in the rostral and caudal regions of the MEE at E14.5, indicating a defect in the TGFbeta signaling pathway

persistence of medial edge epithelium during palatal shelf fusion ( J:248781 )

• although formation and elevation of the palatal shelves is observed in the rostral region, no midline epithelial seam is seen in the caudal region at E14.5 and palatal fusion is incomplete from E15.5 to E16.5

• a significant reduction of apoptosis is detected at the MEE seam at E14.5

• however, palatal edge epithelial cell proliferation is normal

cleft palate ( J:248781 )

• all mice show a cleft palate phenotype

digestive/alimentary system

abnormal secondary palate development ( J:248781 )

• TUNEL assays showed a significant decrease in the number of apoptotic cells in the rostral and caudal regions of the medial edge epithelium (MEE) of the palatal shelves at E14.5 and E15.5

• immunohistochemistry showed a significant reduction of SMAD2/3 phosphorylation in the rostral and caudal regions of the MEE at E14.5, indicating a defect in the TGFbeta signaling pathway

persistence of medial edge epithelium during palatal shelf fusion ( J:248781 )

• although formation and elevation of the palatal shelves is observed in the rostral region, no midline epithelial seam is seen in the caudal region at E14.5 and palatal fusion is incomplete from E15.5 to E16.5

• a significant reduction of apoptosis is detected at the MEE seam at E14.5

• however, palatal edge epithelial cell proliferation is normal

cleft palate ( J:248781 )

• all mice show a cleft palate phenotype