Phenotypes associated with this allele

• Allele Symbol: Clta^tm1.2Fmb
• Allele Name: targeted mutation 1.2, Frances M Brodsky
• Allele ID: MGI:7328974
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Clta^tm1.2Fmb/Clta^tm1.2Fmb	involves: C57BL/6	MGI:7329035

Genotype
MGI:7329035

hm1

• Allelic Composition: Clta^tm1.2Fmb/Clta^tm1.2Fmb
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased survivor rate ( J:296366 )

• mice exhibit a 50% survival rate after weaning

postnatal lethality, incomplete penetrance ( J:235591 )

• about 50% of homozygotes die within a week of birth

immune system

abnormal class switch recombination ( J:235591 )

• mice exhibit an increased frequency of IgA-expressing germinal center B cells in Peyers patches and a variable but, on average, twofold reduction in IgG1-expressing B cells, indicating an enhanced isotype switch to IgA production

decreased germinal center B cell number ( J:235591 )

• mixed bone marrow chimera experiments indicate reduced numbers of germinal center B cells

hematopoietic system

abnormal class switch recombination ( J:235591 )

• mice exhibit an increased frequency of IgA-expressing germinal center B cells in Peyers patches and a variable but, on average, twofold reduction in IgG1-expressing B cells, indicating an enhanced isotype switch to IgA production

decreased germinal center B cell number ( J:235591 )

• mixed bone marrow chimera experiments indicate reduced numbers of germinal center B cells

nervous system

nervous system phenotype ( J:296366 )

N • mice exhibit normal hippocampus morphology at 9-10 months of age

abnormal synaptic vesicle number ( J:296366 )

• size of the readily releasable pool (RRP) of SVs is smaller than that in wild-type controls

• SV density is decreased in excitatory hippocampal neurons, indicating that neurons with only nCLCb clathrin cannot compensate for defective SV regeneration and show reduced SV pools from impaired replenishment

• other parameters, including individual SV size, postsynaptic density length and excitatory synapse density are similar to those in wild-type controls

abnormal synaptic vesicle recycling ( J:296366 )

• in hippocampal neurons, the rate of synaptic vesicle (SV) recycling is decreased, indicating a defect in acute SV pool replenishment

abnormal CNS synaptic transmission ( J:296366 )

• basal synaptic transmission is impaired

decreased excitatory postsynaptic current amplitude ( J:296366 )

• in hippocampal slices, the evoked excitatory postsynaptic current (EPSC) response amplitude is reduced relative to that in wild-type controls

decreased neurotransmitter release ( J:296366 )

• in hippocampal slices, the paired-pulse ratio (PPR) of evoked EPSCs at CA1 synapses is increased, consistent with a reduction in the probability of neurotransmitter release

behavior/neurological

abnormal motor capabilities/coordination/movement ( J:296366 )

• surviving mice show defects in a grid-walking test

• however, grip strength is normal

impaired coordination ( J:296366 )

• surviving mice exhibit impaired motor coordination in an accelerating rotarod test