Phenotypes associated with this allele

• Allele Symbol: Rr253^em1Kmm
• Allele Name: endonuclease-mediated mutation 1, Kenneth M Murphy
• Allele ID: MGI:7331606
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rr253^em1Kmm/Rr253^em1Kmm	C57BL/6-Rr253^em1Kmm	MGI:7331619

Genotype
MGI:7331619

hm1

• Allelic Composition: Rr253^em1Kmm/Rr253^em1Kmm
• Genetic Background: C57BL/6-Rr253^em1Kmm

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:316113 )

N • normal red pulp macrophage (RPM) number

N • normal NK and ILC3 cell numbers in small intestine (SI) lamina propria

N • normal steady-state numbers of lung alveolar and interstitial MFs, liver Kupffer cells, and peritoneal cavity macrophages (PCMs)

N • normal yolk sac macrophage (YS MF) development in E10.5 and fetal liver monocyte (FL MO) and macrophage (FL MF) development in E15.5 embryos

abnormal class switch recombination ( J:316113 )

• impaired CSR to IgG1 after induction by combination of LPS and IL-4

increased neutrophil cell number ( J:316113 )

• as percentage of splenocytes

• normal absolute number

decreased plasmacytoid dendritic cell number ( J:316113 )

• absent plasmacytoid dendritic cells (pDCs)

increased dendritic cell number ( J:316113 )

• type 1 classical dendritic cell (cDC1s) as percentage of splenocytes

• normal absolute number

decreased B cell number ( J:316113 )

• 97% reduction as percentage of splenocytes; 99% reduction in absolute number

decreased mature NK cell number ( J:316113 )

• 84% reduction in maturation in spleen

abnormal effector T cell number ( J:316113 )

• significantly reduced number of lymphocytic choriomeningitis virus Armstrong strain (LCMV-Arm)-specific KLRG1-hi CD127-lo terminal effector CD8+ T cells 8 days after infection

increased lymphocyte cell number ( J:316113 )

• increased innate lymphoid cell type 1 and 2 (ILC1, ILC2) numbers in small intestine (SI) lamina propria

increased NK cell number ( J:316113 )

• as percentage of splenocytes

• normal absolute number

increased T cell number ( J:316113 )

• as percentage of splenocytes

• normal absolute number

decreased monocyte cell number ( J:316113 )

• absent

abnormal peritoneal macrophage morphology ( J:316113 )

• lack of peritoneal cavity macrophage (PCM) regeneration 72 hours after intraperitoneal thioglycolate administration

spleen hypoplasia ( J:316113 )

• 67% reduction in splenic cellularity

abnormal spleen B cell follicle morphology ( J:316113 )

• fewer and smaller

decreased spleen B cell follicle number ( J:316113 )

decreased spleen B cell follicle size ( J:316113 )

immune system

abnormal class switch recombination ( J:316113 )

• impaired CSR to IgG1 after induction by combination of LPS and IL-4

increased neutrophil cell number ( J:316113 )

• as percentage of splenocytes

• normal absolute number

decreased plasmacytoid dendritic cell number ( J:316113 )

• absent plasmacytoid dendritic cells (pDCs)

increased dendritic cell number ( J:316113 )

• type 1 classical dendritic cell (cDC1s) as percentage of splenocytes

• normal absolute number

decreased B cell number ( J:316113 )

• 97% reduction as percentage of splenocytes; 99% reduction in absolute number

decreased mature NK cell number ( J:316113 )

• 84% reduction in maturation in spleen

abnormal effector T cell number ( J:316113 )

• significantly reduced number of lymphocytic choriomeningitis virus Armstrong strain (LCMV-Arm)-specific KLRG1-hi CD127-lo terminal effector CD8+ T cells 8 days after infection

increased lymphocyte cell number ( J:316113 )

• increased innate lymphoid cell type 1 and 2 (ILC1, ILC2) numbers in small intestine (SI) lamina propria

increased NK cell number ( J:316113 )

• as percentage of splenocytes

• normal absolute number

increased T cell number ( J:316113 )

• as percentage of splenocytes

• normal absolute number

decreased monocyte cell number ( J:316113 )

• absent

abnormal peritoneal macrophage morphology ( J:316113 )

• lack of peritoneal cavity macrophage (PCM) regeneration 72 hours after intraperitoneal thioglycolate administration

spleen hypoplasia ( J:316113 )

• 67% reduction in splenic cellularity

abnormal spleen B cell follicle morphology ( J:316113 )

• fewer and smaller

decreased spleen B cell follicle number ( J:316113 )

decreased spleen B cell follicle size ( J:316113 )

increased susceptibility to viral infection induced morbidity/mortality ( J:316113 )

• mice die by 10 days after infection with West Nile Virus

embryo

embryo phenotype ( J:316113 )

N • born at expected Mendelian ratios

mortality/aging

increased susceptibility to viral infection induced morbidity/mortality ( J:316113 )

• mice die by 10 days after infection with West Nile Virus