All Phenotypes Trip6<tm1.2Pher> MGI Mouse

hydrocephaly ( J:312425 )

• 44% of mice develop hydrocephalus

abnormal brain ependyma morphology ( J:312425 )

• mice exhibit defective differentiation of ependyma, showing poorly differentiated epithelial layer comprising atrophic ependymal cells that exhibit epithelial aspects (flattened cell morphology and reduced cytoplasm) and aberrant ciliation

• thinning of the ependyma is also seen in the 3rd and 4th ventricles which do not present with enlargement and ependymal phenotype is seen in both hydrocephalic and non-hydrocephalic mice

• the ependyma epithelial layer only few basal cells

decreased brain ependyma motile cilium length ( J:312425 )

• the percentage of ciliated ependymal cells per ventricular section is reduced by 30%

• the percentage of un-ciliated segments of ependyma is increased 3.4 times

• clusters of cilia that are seen in control ependyma are reduced or eliminated

decreased brain ependyma motile cilium number ( J:312425 )

• cilia length is reduced, with median cilia length reduced by 14%

enlarged lateral ventricles ( J:312425 )

• extension of the lateral ventricles is seen in mice with hydrocephalus

• however, no ventriculomegaly is seen in the 4th ventricle and the 3rd ventricle appears unaffected

• lateral ventricle enlargement is first detected at P1 and maximal extension is reached at P24

abnormal choroid plexus morphology ( J:312425 )

• mice exhibit defective differentiation of choroid plexus with reduced ciliation

• choroid plexus epithelial cells have fewer and shorter cilia