All Phenotypes Rr282729<tm1.1Iku> MGI Mouse

hematopoietic system

abnormal T cell receptor gamma chain V-J recombination ( J:288442 )

• normal Trgv2-Trgj1 and Trgv5-Trgj1 rearrangements in adult TN thymocytes

• absent Tcrg-V1.1-Trgj4 rearrangements in adult TN thymocytes and E16.5 embryo thymus

• 90% reduced Tcrg-V1.2-Trgj2 rearrangements in adult TN thymocytes and E16.5 embryo thymus

• normal Trgv2-Trgj1, Trgv3-Trgj1 and Trgv4-Trgj1 rearrangements in E16.5 embryo thymus

decreased gamma-delta T cell number ( J:288442 )

• reduced number of Tcrg-V1.1+ cells and Trgv5+ cells in adult thymus

• reduced number of Tcrg-V1.1- Trgv2- Trgv3- Trgv5- cells in adult thymus

• reduced number of Tcrg-V1.1+ cells in E16.5 embryo thymus

• 60-70% reduced in spleen and lymph nodes, with Tcrg-V1.1+ cells virtually absent and 40% decrease in number and frequency of Trgv2+ cells

• 80% reduction amongst small intestinal intraepithelial lymphocytes (siIELs), with Tcrg-V1.1+ cells absent and 85-90% decrease in number of Trgv5+ cells

• reduced number and frequency of CD27+ Trgv2+ cells in spleen and lymph nodes

• reduced number of CD27+ CD45RB-high Trgv2+ cells in spleen, lymph nodes and peripheral blood

• reduced number of Ly6C+ CD44-high Trgv2+ cells in in lymph nodes

• normal number of CD27+ CD45RB-high Trgv2+ cells in spleen

• normal number of CD27+ CD45RB-low Trgv2+ cells in spleen, lymph nodes and peripheral blood

• normal number of CD27- Trgv2+ cells in spleen and lymph nodes

• normal number and frequency of Trgv3+ cells (dendritic epidermal cells (DETCs)) in adult ear skin

• normal number of Trgv2+ cells and Trgv3+ cells in E16.5 embryo thymus

• normal number of Tcrg-V1.1- Trgv2- Trgv3- Trgv5- cells in E16.5 embryo thymus

• normal total number of thymocytes in adult thymus and gamma-delta T cell numbers in E16.5 embryo thymus

• normal number of Trgv2+ cells in adult thymus

immune system