Phenotypes associated with this allele

• Allele Symbol: Cep72^em1Lmgg
• Allele Name: endonuclease-mediated mutation 1, Mengcheng Luo
• Allele ID: MGI:7398570
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cep72^em1Lmgg/Cep72^em1Lmgg	C57BL/6N-Cep72^em1Lmgg	MGI:7398572

Genotype
MGI:7398572

hm1

• Allelic Composition: Cep72^em1Lmgg/Cep72^em1Lmgg
• Genetic Background: C57BL/6N-Cep72^em1Lmgg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:330932 )

N • females are fertile with no defects in follicular development relative to control females

N • despite abnormal spermatozoa morphology, males are fertile with normal testis to body weight ratio, testis and epididymal histology and caudal epididymal sperm count, and no defects in synaptonemal complex formation or meiotic prophase I procession in spermatocytes relative to control males

teratozoospermia ( J:330932 )

♂ • males show a marked increase in the number of morphologically abnormal spermatozoa relative to controls (34.94% vs 20.44%), classified into 4 types (type I-IV)

♂ • % of types II and IV is significantly increased relative to controls (67.57% vs 11%)

abnormal sperm flagellum morphology ( J:330932 )

♂ • rate of sperm flagellum structural anomalies is significantly increased relative to controls (13.51% vs. 6.53%)

abnormal outer dense fiber morphology ( J:330932 )

♂ • some sperm show partial absence of outer dense fibers (ODF) in the principal piece

abnormal sperm axoneme morphology ( J:330932 )

♂ • some sperm show partial absence of the central pair microtubules (CP) and outer doublet microtubules (OD) in the principal piece

abnormal sperm principal piece morphology ( J:330932 )

♂ • some sperm show partial absence of outer dense fibers (ODF), central pair microtubules (CP) and outer doublet microtubules (OD) in the principal piece

coiled sperm flagellum ( J:330932 )

♂ • type IV sperm have no head and curved flagellum

abnormal sperm head morphology ( J:330932 )

♂ • type I sperm display abnormal head shape with PNA (acrosome marker) and DAPI (nucleus marker) staining

♂ • type II sperm display rod head with no acrosome and nucleus

♂ • type III sperm show hollow head with no acrosome and nucleus

absent acrosome ( J:330932 )

♂ • type II sperm display rod head with no acrosome and nucleus

♂ • type III sperm show hollow head with no acrosome and nucleus

abnormal sperm nucleus morphology ( J:330932 )

♂ • type II sperm display rod head with no acrosome and nucleus

♂ • type III sperm show hollow head with no acrosome and nucleus

absent sperm head ( J:330932 )

♂ • type IV sperm have no head and curved flagellum

abnormal spermiogenesis ( J:330932 )

♂

cellular

teratozoospermia ( J:330932 )

♂ • males show a marked increase in the number of morphologically abnormal spermatozoa relative to controls (34.94% vs 20.44%), classified into 4 types (type I-IV)

♂ • % of types II and IV is significantly increased relative to controls (67.57% vs 11%)

abnormal sperm flagellum morphology ( J:330932 )

♂ • rate of sperm flagellum structural anomalies is significantly increased relative to controls (13.51% vs. 6.53%)

abnormal outer dense fiber morphology ( J:330932 )

♂ • some sperm show partial absence of outer dense fibers (ODF) in the principal piece

abnormal sperm axoneme morphology ( J:330932 )

♂ • some sperm show partial absence of the central pair microtubules (CP) and outer doublet microtubules (OD) in the principal piece

abnormal sperm principal piece morphology ( J:330932 )

♂ • some sperm show partial absence of outer dense fibers (ODF), central pair microtubules (CP) and outer doublet microtubules (OD) in the principal piece

coiled sperm flagellum ( J:330932 )

♂ • type IV sperm have no head and curved flagellum

abnormal sperm head morphology ( J:330932 )

♂ • type I sperm display abnormal head shape with PNA (acrosome marker) and DAPI (nucleus marker) staining

♂ • type II sperm display rod head with no acrosome and nucleus

♂ • type III sperm show hollow head with no acrosome and nucleus

absent acrosome ( J:330932 )

♂ • type II sperm display rod head with no acrosome and nucleus

♂ • type III sperm show hollow head with no acrosome and nucleus

abnormal sperm nucleus morphology ( J:330932 )

♂ • type II sperm display rod head with no acrosome and nucleus

♂ • type III sperm show hollow head with no acrosome and nucleus

absent sperm head ( J:330932 )

♂ • type IV sperm have no head and curved flagellum