Phenotypes associated with this allele

• Allele Symbol: Zfp746^tm1.1Tmd
• Allele Name: targeted mutation 1.1, Ted M Dawson
• Allele ID: MGI:7428903
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zfp746^tm1.1Tmd/Zfp746^tm1.1Tmd	involves: 129S4/SvJae	MGI:7764027
cx2	Tg(tetO/Prnp-SNCA*A53T)4360Tmd/0 Zfp746^tm1.1Tmd/Zfp746^tm1.1Tmd	involves: 129S4/SvJae * C3H * C57BL/6	MGI:7764517
cx3	2310039L15Rik^Tg(Prnp-SNCA*A53T)23Mkle/2310039L15Rik^+ Zfp746^tm1.1Tmd/Zfp746^tm1.1Tmd	involves: 129S4/SvJae * C3H/HeJ * C57BL/6J	MGI:7764514

Genotype
MGI:7764027

hm1

• Allelic Composition: Zfp746^tm1.1Tmd/Zfp746^tm1.1Tmd
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:327648 )

• mice are viable and live a normal life-span, exhibit normal body weight and no gross developmental or behavioral defects, no obvious abnormalities in heart, lung, spleen, kidney, thymus. liver, intestine, gonad, eye or cerebrum, or in hippocampal or dopaminergic neurons or in dopamine levels or its metabolites

• mice injected with alpha-synuclein preformed fibril into the dorsal striatum show rescue of the alpha-synuclein preformed fibril-induced loss of dopamine neurons, striatal deficits of dopamine and its metabolites DOPAC and HVA, and reduction in dopamine fiber density, prevention of the behavioral abnormalities in the Pole test, the loss of grip strength

Genotype
MGI:7764517

cx2

• Allelic Composition: Tg(tetO/Prnp-SNCA*A53T)4360Tmd/0; Zfp746^tm1.1Tmd/Zfp746^tm1.1Tmd
• Genetic Background: involves: 129S4/SvJae * C3H * C57BL/6

nervous system

nervous system phenotype ( J:327648 )

N • mice injected with an adenovirus expressing tTA into the ventral midbrain show rescue of the dopamine neuron degeneration, loss of dopamine, and the loss of striatal dopamine fiber density, the prevention of amphetamine-induced rotation, and rescue of GFAP immunoreactivity seen in single Tg(tetO/Prnp-SNCA*A53T)4360Tmd/0 mice

Genotype
MGI:7764514

cx3

• Allelic Composition: 2310039L15Rik^{Tg(Prnp-SNCA*A53T)23Mkle}/2310039L15Rik⁺; Zfp746^tm1.1Tmd/Zfp746^tm1.1Tmd
• Genetic Background: involves: 129S4/SvJae * C3H/HeJ * C57BL/6J

mortality/aging

extended life span ( J:327648 )

• mice live an average of 15 months, which indicates prolonged survival by 5 months compared to single 2310039L15Rik^{Tg(Prnp-SNCA*A53T)23Mkle} heterozygotes

behavior/neurological

decreased vertical activity ( J:327648 )

• mice show a reduction in vertical activity in the open field at 6 months of age compared to single 2310039L15Rik^{Tg(Prnp-SNCA*A53T)23Mkle} heterozygotes

decreased locomotor activity ( J:327648 )

• mice show a reduction in horizontal activity in the open field at 6 months of age compared to single 2310039L15Rik^{Tg(Prnp-SNCA*A53T)23Mkle} heterozygotes