Phenotypes associated with this allele

• Allele Symbol: Polr2j^em1(IMPC)Bay
• Allele Name: endonuclease-mediated mutation 1, Baylor College of Medicine
• Allele ID: MGI:7447412
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Polr2j^em1(IMPC)Bay/Polr2j^em1(IMPC)Bay	C57BL/6NJ-Polr2j^em1(IMPC)Bay/Mmmh	MGI:7734650
hm2	Polr2j^em1(IMPC)Bay/Polr2j^em1(IMPC)Bay	C57BL/6N-Polr2j^em1(IMPC)Bay/BayMmucd	MGI:7629078

Genotype
MGI:7734650

hm1

• Allelic Composition: Polr2j^em1(IMPC)Bay/Polr2j^em1(IMPC)Bay
• Genetic Background: C57BL/6NJ-Polr2j^em1(IMPC)Bay/Mmmh

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Polr2j^em1(IMPC)Bay/Polr2j^em1(IMPC)Bay mice exhibit embryonic lethality, with embryos recovered at E3.5 but not at E7.5. Embryos at E3.5 appear as dying morulae or blastocysts. Mutants fail to hatch from the zona pellucida and die after 3 days in culture.

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:279207 )

• homozygous embryos are present in expected Mendelian ratios at E3.5 and appear as dying morulae or blastocysts; no homozygous embryos or excess resorption sites are observed at E7.5

embryo

failure of blastocyst to hatch from the zona pellucida ( J:279207 )

• in vitro outgrowth assays show that embryos fail to hatch from the zona pellucida after 3 days in culture

abnormal morula morphology ( J:279207 )

• some embryos recovered at E3.5 appear as dying morulae

reproductive system

failure of embryo implantation ( J:279207 )

• in vitro, embryos fail to hatch and are dead after 3 days in culture

Genotype
MGI:7629078

hm2

• Allelic Composition: Polr2j^em1(IMPC)Bay/Polr2j^em1(IMPC)Bay
• Genetic Background: C57BL/6N-Polr2j^em1(IMPC)Bay/BayMmucd

Data Sources

IMPC Data for Polr2j

mortality/aging

preweaning lethality, complete penetrance ( J:211773 )

IMPC - BCM