All Phenotypes Tssk3<em1Amsa> MGI Mouse

reproductive system phenotype ( J:334491 )

N	• female homozygotes are fertile with pregnancy rates and litter size comparable to wild-type controls • male homozygotes show no alterations in epididymis size, testis morphology and weight, or seminiferous tubule diameter relative to wild-type controls

oligozoospermia ( J:334491 )

• number of mature sperm obtained from the cauda epididymis is drastically reduced

teratozoospermia ( J:334491 )

• few mature sperm recovered show severe morphological defects, including malformed heads or broken tails

• less than 10% of total number of sperm counted are normal

• broken sperm tails are observed

• cross sections of sperm flagella show loss of outer dense fiber components

abnormal sperm axoneme morphology ( J:334491 )

• cross sections of sperm flagella show loss of axoneme components

abnormal sperm head morphology ( J:334491 )

• malformed heads are observed

detached acrosome ( J:334491 )

• detached acrosomes are detected

immotile sperm ( J:334491 )

• few mature sperm recovered are completely immotile

abnormal spermiogenesis ( J:334491 )

• round spermatids positive for the acrosomal marker peanut agglutinin (PNA) are present in the cauda epididymis

• in testis sections, step 15 and 16 elongated spermatids exhibit an abnormal organization pattern

arrest of spermatogenesis ( J:334491 )

• spermatogenesis is blocked at the VII/VIII stage

male infertility ( J:334491 )

• male homozygotes are unable to impregnate wild-type females upon mating

• however, mating behavior is normal, as determined by the presence of copulatory plugs