Analysis Tools
vision/eye
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• at P60, lens epithelial cells show extensive vacuolization
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• at P60, lens fiber cells are large and display irregular shapes
• at P0, P7, and P14, differentiating lens fiber cells show abnormal retention of cellular organelles, including endoplasmic reticulum (ER), mitochondria, and Golgi apparatus (GA), suggesting impaired organelle removal
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• mice show initial signs of lens opacities at 4 weeks that develop into mild cataracts at ~8 weeks of age
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• mice develop mature bilateral cataracts by 16 weeks of age; extent of lens opacities is variable among mice
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cellular
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• at P14, lenses show an increased Golgi apparatus mass relative to age-matched wild-type lenses
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• at P0, P7, P14 and P21, lenses show an increased ER mass relative to age-matched wild-type lenses
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• at P0, lenses show alterations in many autophagy-associated genes, proteins and lipids, including a 1.48-fold increase in the level of SQSTM1/p62 and a modest accumulation of phosphatidylethanolamine (PE) phospholipids, suggesting impaired autophagy
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homeostasis/metabolism
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• at P0, lenses show alterations in many autophagy-associated genes, proteins and lipids, including a 1.48-fold increase in the level of SQSTM1/p62 and a modest accumulation of phosphatidylethanolamine (PE) phospholipids, suggesting impaired autophagy
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• at P0, lenses show a modest accumulation of phosphatidylethanolamine (PE) phospholipids
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