Phenotypes associated with this allele

• Allele Symbol: Jade2^em1Cya
• Allele Name: endonuclease-mediated mutation 1, Cyagen Biosciences
• Allele ID: MGI:7482646
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Jade2^em1Cya/Jade2^em1Cya	involves: C57BL/6	MGI:7518882

Genotype
MGI:7518882

hm1

• Allelic Composition: Jade2^em1Cya/Jade2^em1Cya
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased dendritic spine density ( J:333965 )

• hippocampal neurons show a decrease in spine density

• mice injected with an adenovirus expressing RAC1 (AAV-RAC1) show almost normal spine density of hippocampal neurons

impaired synaptic plasticity ( J:333965 )

• NMDA receptor/AMPA receptor ratio is decreased, indicating abnormal hippocampal synaptic plasticity

abnormal synaptic transmission ( J:333965 )

• mice show impaired basal synaptic transmission

• however, paired-pulse ratios in hippocampal CA1 pyramidal neurons are normal suggesting normal presynaptic neurotransmitter release

abnormal excitatory postsynaptic potential ( J:333965 )

• downward shift of input-output curves of field excitatory postsynaptic potentials as a function of stimulus intensity

reduced long-term potentiation ( J:333965 )

• long-term potentiation induced by high-frequency stimulation in the hippocampus is reduced

• mice injected with AAV-RAC1 into the CA1 region reverses the long-term potentiation to nearly wild-type levels

decreased miniature excitatory postsynaptic current frequency ( J:333965 )

• mEPSC frequency in CA1 pyramidal neurons is reduced

• injection of AAV-RAC1 into the CA1 region reverses the mEPSC frequency to almost normal levels

• however, no effect on neuronal intrinsic excitability and mIPSC properties is seen

behavior/neurological

impaired long-term object recognition memory ( J:333965 )

• mice show impaired recognition memory in the novel object recognition test

• AAV-RAC1 injection into the CA1 region alleviates this impairment

abnormal long-term spatial reference memory ( J:333965 )

• mice show a longer time to find the hidden platform and a shorter time of staying in the target quadrant in the Morris water maze test, with normal swimming speed

• AAV-RAC1 injection into the CA1 region rescues the spatial memory deficits