Phenotypes associated with this allele

• Allele Symbol: Cul3^em1Limi
• Allele Name: endonuclease-mediated mutation 1, Lilia M Iakoucheva
• Allele ID: MGI:7491948
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cul3^em1Limi/Cul3^em1Limi	C57BL/6N-Cul3^em1Limi	MGI:7492273
ht2	Cul3^em1Limi/Cul3^+	C57BL/6N-Cul3^em1Limi	MGI:7492275

Genotype
MGI:7492273

hm1

• Allelic Composition: Cul3^em1Limi/Cul3^em1Limi
• Genetic Background: C57BL/6N-Cul3^em1Limi

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:332937 )

• no mice born

Genotype
MGI:7492275

ht2

• Allelic Composition: Cul3^em1Limi/Cul3⁺
• Genetic Background: C57BL/6N-Cul3^em1Limi

behavior/neurological

behavior/neurological phenotype ( J:332937 )

N • no difference in time spent in center versus periphery in open field test

N • normal auditory startle and forelimb grasping milestones

abnormal fear/anxiety-related behavior ( J:332937 )

• longer time to reach cliff avoidance milestone

abnormal response to novel object ( J:332937 )

• shorter time spent investigating novel object in novel object recognition test

abnormal response to social novelty ( J:332937 )

• less time spent sniffing novel mouse in three-chamber test with novel object in the other chamber

• same time spent sniffing novel and familiar mouse in three-chamber test

impaired righting response ( J:332937 )

• longer time to reach surface righting milestone

hyperactivity ( J:332937 )

♂ • longer distance traveled and higher speed in open field test

nervous system

abnormal brain morphology ( J:332937 )

• elongated brain in adults

• reduced relative gray matter volume at age 11 weeks

• increased relative volume of some subcortical regions at age 11 weeks

• normal relative white matter volume at age 11 weeks

decreased brain size ( J:332937 )

• reduced total brain volume at ages P7 and 11 weeks

decreased corpus callosum size ( J:332937 )

• reduced relative volume at age 11 weeks

hypothalamus hyperplasia ( J:332937 )

• at age P7

abnormal entorhinal cortex morphology ( J:332937 )

• reduced relative volume at age 11 weeks

decreased dentate gyrus size ( J:332937 )

• reduced relative volume at age 11 weeks

abnormal somatosensory cortex morphology ( J:332937 )

• reduced relative volume at age 11 weeks

abnormal visual cortex morphology ( J:332937 )

• reduced relative volume of primary visual cortex at age 11 weeks

thin cerebral cortex ( J:332937 )

• at age 11 weeks

• reduced frontal and parieto-temporal cortex thickness at age P7

small olfactory bulb ( J:332937 )

• reduced relative volume at age 11 weeks

small cerebellum ( J:332937 )

• reduced relative volume at ages P7 and 11 weeks

abnormal dendrite morphology ( J:332937 )

• reduced number of neurites on primary cortical neurons from E17.5 embryos

decreased dendritic spine length ( J:332937 )

• on primary cortical neurons from E17.5 embryos

abnormal nervous system electrophysiology ( J:332937 )

• reduced weighted mean firing rate of cultured primary cortical neurons from E17.5 embryos

cellular

abnormal actin cytoskeleton morphology ( J:332937 )

• reduced stability and reduced number of F-actin puncta per dendrite length in cultured adult primary cortical neurons

embryo

embryo phenotype ( J:332937 )

N • viable

decreased embryo size ( J:332937 )

• in E15.5 and E17.5 embryos

growth/size/body

decreased embryo size ( J:332937 )

• in E15.5 and E17.5 embryos

decreased body size ( J:332937 )

• in adults

decreased body weight ( J:332937 )

• in adults

mortality/aging

mortality/aging ( J:332937 )

N • normal lifespan

N • viable

craniofacial

craniofacial phenotype ( J:332937 )

N • normal ear unfolding milestone

reproductive system

reproductive system phenotype ( J:332937 )

N • fertile

vision/eye

vision/eye phenotype ( J:332937 )

N • normal eye opening milestone