Phenotypes associated with this allele

• Allele Symbol: Rhoq^{tm1b(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:7492305
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rhoq^tm1b(EUCOMM)Hmgu/Rhoq^tm1b(EUCOMM)Hmgu	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	MGI:7525601

Genotype
MGI:7525601

hm1

• Allelic Composition: Rhoq^{tm1b(EUCOMM)Hmgu}/Rhoq^{tm1b(EUCOMM)Hmgu}
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:308705 )

N • at P56, Kluver-Barrera-stained coronal brain sections show no differences in normalized axon tract length in the corpus callosum and anterior commissure relative to wild-type brains

abnormal axon extension ( J:308705 )

• at 2.5 days in culture, hippocampal neurons show significantly reduced axon elongation; axon length is 37% shorter than that in wild-type neurons

• after 1.5 days in vitro, live axons display a significantly higher average number of retraction events during the 10 hr imaging period than wild-type axons

• however, axon polarization is normal with no significant difference in the level of insulin-like growth factor-1 receptor (IGF1R) enrichment in future axon tips

abnormal neurite morphology ( J:308705 )

• at 2.5 days in culture, hippocampal neurons show a reduction in the average length of the minor (non-longest) neurites relative to wild-type neurons

• however, the average number of neurites is normal at 60 hr in culture

abnormal axon morphology ( J:308705 )

• at 2 days in culture, hippocampal neurons exhibit more splayed and less bundled microtubules at the growth cone neck and have axon growth cone areas that are 40% smaller than those of wild-type axons

impaired central nervous system regeneration ( J:308705 )

• at 2 weeks after optic nerve crush (ONC), mice show a significantly lower number of regenerated GAP43-positive retinal ganglion cell (RGC) axons than control mice up to 1.5 mm distal to the crush site

• however, the survival rate of RGCs after ONC is similar to that in wild-type controls

abnormal peripheral nervous system regeneration ( J:308705 )

• mice show defective motor axon regeneration in injured hypoglossal nerves; at 4 weeks after axotomy, only ~10% of motor neurons are Fluoro-Gold (FG)-positive (regenerated) in injured hypoglossal nuclei versus ~60% FG-positive (regenerated) cells in wild-type controls

• however, decrease in FG-positive cell number is not due to death of injured motor neurons as ~70% of neurons survive for 4 weeks after hypoglossal nerve axotomy, as seen in wild-type controls

cellular

abnormal microtubule cytoskeleton morphology ( J:308705 )

• at 2 days in culture, hippocampal neurons exhibit more splayed and less bundled microtubules at the growth cone neck and have axon growth cone areas that are 40% smaller than those of wild-type axons

abnormal axon extension ( J:308705 )

• at 2.5 days in culture, hippocampal neurons show significantly reduced axon elongation; axon length is 37% shorter than that in wild-type neurons

• after 1.5 days in vitro, live axons display a significantly higher average number of retraction events during the 10 hr imaging period than wild-type axons

• however, axon polarization is normal with no significant difference in the level of insulin-like growth factor-1 receptor (IGF1R) enrichment in future axon tips