Analysis Tools
reproductive system
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• 6-week-old female mice exhibit normal ovary histology and ovary/body weight ratio; folliculogenesis is induced by hormone (eCG) stimulation, suggesting normal oocyte growth
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• nearly all oocytes arrest at the germinal vesicle (GV) stage without undergoing nuclear envelope breakdown or completing meiosis
• many poly(A) mRNAs are transformed into uridylated-poly(A) mRNAs which are more stable, resulting in global accumulation of transcripts
• uridylated-poly(A) RNAs are stabilized due to insufficient degradation and tail editing
• insufficient degradation of meiotic repressor transcripts may cause GV arrest
• however, the diameter of GV oocytes and the ratio between surrounded nucleolus (SN) and non-surrounded nucleolus (NSN) oocytes are similar to those of control oocytes
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• transcriptome analysis during meiotic maturation of oocytes showed that the transition from GV to meiosis II (MII) stages is perturbed
• oocytes show a severe and early arrest in transitioning from meiosis I prophase to metaphase
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• female mice are completely infertile
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cellular
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• nearly all oocytes arrest at the germinal vesicle (GV) stage without undergoing nuclear envelope breakdown or completing meiosis
• many poly(A) mRNAs are transformed into uridylated-poly(A) mRNAs which are more stable, resulting in global accumulation of transcripts
• uridylated-poly(A) RNAs are stabilized due to insufficient degradation and tail editing
• insufficient degradation of meiotic repressor transcripts may cause GV arrest
• however, the diameter of GV oocytes and the ratio between surrounded nucleolus (SN) and non-surrounded nucleolus (NSN) oocytes are similar to those of control oocytes
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• transcriptome analysis during meiotic maturation of oocytes showed that the transition from GV to meiosis II (MII) stages is perturbed
• oocytes show a severe and early arrest in transitioning from meiosis I prophase to metaphase
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• a small cohort of mRNAs are polyadenylated after terminal uridylation in germinal vesicle (GV) oocytes; these uridylated-poly(A) RNAs have shorter poly(A) tails and show reduced translation activity in oocytes
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homeostasis/metabolism
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• a small cohort of mRNAs are polyadenylated after terminal uridylation in germinal vesicle (GV) oocytes; these uridylated-poly(A) RNAs have shorter poly(A) tails and show reduced translation activity in oocytes
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