All Phenotypes Tssk3<em1Osb> MGI Mouse

abnormal male germ cell morphology ( J:337565 )

• testes show significant vacuolization of male germ cells

• defects in spermiogenesis and spermiation result in degeneration and rapid reabsorption of elongating/elongated spermatids in testes

abnormal male germ cell physiology ( J:337565 )

• phosphoproteomics analysis of purified testicular germ cells showed significantly reduced phosphorylation of multiple infertility-related proteins, including GAPDHS, ACTL7A, ACTL9 and REEP6

• however, total protein levels remain unaffected

abnormal seminiferous tubule epithelium morphology ( J:337565 )

• all stages of the seminiferous epithelium cycle (I-XII) show some form of disorganization; most tubule cross-sections contain germ cells that appear to be either too early or too late for that stage

• however, both testis size and weight are normal

seminiferous tubule degeneration ( J:337565 )

• ~28.7% of seminiferous tubule cross-sections exhibit vacuolization versus only ~1.5% in controls

• omitting stages VII and VIII, ~92% of all circular tubule cross-sections that contain vacuolization are at stages IV-VI and ~8% are at stage IX

abnormal spermatogenesis ( J:337565 )

• histological and ultrastructural analysis showed obvious defects in late spermatogenesis

oligozoospermia ( J:337565 )

• caudal epididymal sperm count is nearly ablated relative to that in heterozygous control males

• epididymis histology revealed only a few sperm present in the lumen

teratozoospermia ( J:337565 )

• cauda epididymal sperm appear morphologically abnormal

abnormal sperm midpiece morphology ( J:337565 )

• all of the few spermatozoa retrieved from the epididymis exhibit thin sperm midpieces

abnormal sperm head morphology ( J:337565 )

• all of the few spermatozoa retrieved from the epididymis exhibit amorphous head morphology

abnormal acrosome morphology ( J:337565 )

• at steps 14 and 15, spermatids show unstructured acrosome morphology and degradation

detached acrosome ( J:337565 )

• acrosomes are detached from the sperm nuclei

abnormal manchette morphology ( J:337565 )

• at steps 14 and 15, spermatids show unstructured manchette morphology and degradation

abnormal spermiogenesis ( J:337565 )

• step 14-15 spermatids exhibit excess cytoplasm around normal-shaped nuclei

• early elongating spermatids that are typical of stage IX are instead observed at XII

• elongating spermatids that should have been fully developed at stage I appear underdeveloped

• elongated spermatids do not line the lumen at stage VII; stages VII and VIII are difficult to ascertain through standard staging criteria

• elongated spermatids fail to proceed normally along the spermiation pathway, resulting in a higher distribution of stages I-VI

• however, tubules progress to stage IX as irregularly shaped round spermatids can be found

abnormal spermiation ( J:337565 )

• elongated spermatids that should have undergone spermiation at stage VIII are frequently observed at later stages IX-XI, with unreleased fully elongated spermatids still embedded in the epithelium

male infertility ( J:337565 )

• male mice fail to sire pups when mated with wild-type females for >4 months, indicating complete male sterility