Phenotypes associated with this allele

• Allele Symbol: Zfp276^{tm1c(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:7510125
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Zfp276^tm1c(EUCOMM)Hmgu/Zfp276^tm1c(EUCOMM)Hmgu Tg(Sox10-cre)1Wdr/0	involves: C57BL/6 * C57BL/6N * CBA	MGI:7619747

Genotype
MGI:7619747

cn1

• Allelic Composition: Zfp276^{tm1c(EUCOMM)Hmgu}/Zfp276^{tm1c(EUCOMM)Hmgu}; Tg(Sox10-cre)1Wdr/0
• Genetic Background: involves: C57BL/6 * C57BL/6N * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal oligodendrocyte differentiation ( J:324574 )

• mice show a transient delay in oligodendrocytes (OL) maturation and myelination: cells expressing Myrf protein (an OL maturity marker) in transverse spinal cord sections are reduced to 56% of controls at P7, with similar decreases noted in cells expressing myelin genes Mbp and Plp1 and Gstpi protein; at P30 only a slight reduction in Gstpi numbers is still observed, whereas no differences in all other differentiation markers and myelin genes are detected at P30 or P60

• however, overall numbers of Sox10+ oligodendroglial cells (OGCs), Pdgfra+ oligodendrocyte progenitor cells (OPCs), Gfap+ astrocytes, and Iba1+ microglia are not significantly changed in the spinal cord at P1-P60

abnormal myelination ( J:324574 )

• cells expressing myelin genes Mbp and Plp1 and Gstpi protein are significantly reduced in transverse spinal cord sections at P7; however, only a slight reduction in Gstpi numbers is still observed at P30

• analyses of spinal cord white matter regions showed an increased g-ratio and a drastically reduced number of myelinated axons at P7 but not at P30, indicating a transient delay in the myelination of axons in early postnatal stages

dysmyelination ( J:324574 )

• mice show strongly reduced expression of myelin genes in the spinal cord at P7

• in vitro, RNA-Seq analyses of primary OGC cultures derived from P0-P3 forebrains show significant downregulation of myelination-related genes, consistent with the delayed oligodendrocyte differentiation and hypomyelination phenotype seen in vivo

homeostasis/metabolism

decreased lipogenesis ( J:324574 )

• RNA-Seq analyses of primary OGC cultures derived from P0-P3 forebrains show significant downregulation of genes associated with fatty acid, cholesterol and sphingomyelin biosynthesis