Phenotypes associated with this allele

• Allele Symbol: Caln1^em1Mrkr
• Allele Name: endonuclease-mediated mutation 1, Michael Kreutz
• Allele ID: MGI:7510163
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Caln1^em1Mrkr/Caln1^em1Mrkr	C57BL/6J-Caln1^em1Mrkr	MGI:7532514
cx2	Caln1^em1Mrkr/Caln1^em1Mrkr Tg(Syn1-Cabp7/mCherry)1Mrkr/0	involves: C57BL/6J	MGI:7532516

Genotype
MGI:7532514

hm1

• Allelic Composition: Caln1^em1Mrkr/Caln1^em1Mrkr
• Genetic Background: C57BL/6J-Caln1^em1Mrkr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:338143 )

N • mice show no major alterations in gross hippocampus and cerebellum structure or in the thickness of the different layers of the hippocampus CA1 region

abnormal hippocampus stratum lacunosum morphology ( J:338143 )

• mice show deficits in polysialylation of locally secreted NCAM in distal dendrites of the stratum lacunosum moleculare (SLM)

• however, levels of NCAM and of major polysialyltransferases involved in NCAM polysialylation are normal, suggesting that reduced PSA-NCAM levels in distal dendrites of the SLM are likely due to a reduced number of Golgi satellites

abnormal hippocampus neuron morphology ( J:338143 )

• in culture, primary hippocampal neurons show a significant reduction in Golgi length and a significantly lower % of neurons with extended Golgi than wild-type neurons

abnormal hippocampus pyramidal cell morphology ( J:338143 )

• pyramidal cells of the CA1 region show a mild non-significant reduction in Golgi length that is largely due to a decreased number of neurons endowed with an extended Golgi

• however, no major changes in the morphology of the somatic Golgi are observed

decreased dendritic spine density ( J:338143 )

• following delivery of two viruses (AAV9-EF1alpha-LoxP-EGFP-LoxP and AAV9-CaMKII-Cre) to the CA1 hippocampal region, pyramidal neurons show a significant reduction in spine density toward distal dendrites in the SLM but not in other strata

abnormal hippocampal pyramidal neuron dendrite morphology ( J:338143 )

• CA1 pyramidal neurons show significantly reduced NCAM polysialylation levels in distal dendrites of the stratum radiatum distal (SRD) and stratum lacunosum moleculare (SLM)

impaired synaptic plasticity ( J:338143 )

• mice exhibit impaired long-term synaptic plasticity in distal dendrites of temporoammonic connections to CA1-SLM

abnormal excitatory postsynaptic potential ( J:338143 )

• fEPSP amplitude is reduced by 40% whether in response to stimulation of the Schaffer collateral-CA1 pathway (SC-CA1) or in response to stimulation of the temporoammonic-CA1 (TA-CA1) pathway, indicating deficits in basal transmission in proximal and distal dendrites

• however, paired-pulse facilitation is normal

reduced long-term potentiation ( J:338143 )

• LTP induction in the TA-CA1 pathway to dendrites in the SLM is significantly impaired whereas LTP induction in the SC-CA1 pathway is normal, indicating a selective deficit in LTP at distal but not at more proximal dendrites of CA1 neurons

Genotype
MGI:7532516

cx2

• Allelic Composition: Caln1^em1Mrkr/Caln1^em1Mrkr; Tg(Syn1-Cabp7/mCherry)1Mrkr/0
• Genetic Background: involves: C57BL/6J

nervous system

abnormal hippocampus stratum lacunosum morphology ( J:338143 )

• CA1 pyramidal neurons show a significant reduction in Golgi satellite abundance in distal dendrites of the stratum lacunosum moleculare (SLM)

abnormal hippocampal pyramidal neuron dendrite morphology ( J:338143 )

• CA1 pyramidal neurons show a gradual reduction in Golgi satellite abundance toward more distal dendrites that is statistically significant in SLM