Analysis Tools
reproductive system
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• epididymal sperm show various flagellar abnormalities; sperm with a large vesicle at the midpiece, sperm aggregated into bundles, and sperm with unsheathed flagella at the junction of principal and midpiece piece
• the large vesicle at the midpiece shows electron dense materials, disorganized mitochondria, and a single, large vacuole, suggesting abnormal retention of cytoplasmic remnants and failed cytoplasmic clearance
• 11.97% of sperm aggregate into bundles
• several sperm flagella are wrapped in one cell membrane
• axonemal microtubules and outer dense fibers are extruded from the midpiece and unsheathed flagella at the junction of the principal and midpiece piece which is not seen in wild-type spermatozoa
• however, sperm head shape and acrosome development are normal
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• spermatozoa flagella lacks the outer dense fiber that corresponds to the missing microtubule doublet 7
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• spermatozoa flagella axoneme lacks microtubule doublet 7 and the corresponding outer dense fiber
• 11.64% of flagella axonemes do not have the 7th microtubule doublet in spermatozoa in the testes while absence of the 7th microtubule doublet is seen in 88.77% of caudal epididymis sperm
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• 8.31% of sperm have unsheathed flagella at their principal piece
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• 41.43% of sperm have hairpin-like structures in their midpieces
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• fewer spermatozoa are seen in the caudal epididymis
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• no progressive sperm are seen
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• sperm show decreased motility and only 7.667% of sperm being motile compared to 82.33% of wild-type sperm
• mean path velocity and straight-line velocity of sperm are reduced
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• no pups are born from pairings that include homozygous males
• however, testis and epididymis size, testis weight, body weight, and testis weight to body weight ratio are normal and females are fertile with normal ovaries
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cellular
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• epididymal sperm show various flagellar abnormalities; sperm with a large vesicle at the midpiece, sperm aggregated into bundles, and sperm with unsheathed flagella at the junction of principal and midpiece piece
• the large vesicle at the midpiece shows electron dense materials, disorganized mitochondria, and a single, large vacuole, suggesting abnormal retention of cytoplasmic remnants and failed cytoplasmic clearance
• 11.97% of sperm aggregate into bundles
• several sperm flagella are wrapped in one cell membrane
• axonemal microtubules and outer dense fibers are extruded from the midpiece and unsheathed flagella at the junction of the principal and midpiece piece which is not seen in wild-type spermatozoa
• however, sperm head shape and acrosome development are normal
|
|
|
• spermatozoa flagella lacks the outer dense fiber that corresponds to the missing microtubule doublet 7
|
|
|
• spermatozoa flagella axoneme lacks microtubule doublet 7 and the corresponding outer dense fiber
• 11.64% of flagella axonemes do not have the 7th microtubule doublet in spermatozoa in the testes while absence of the 7th microtubule doublet is seen in 88.77% of caudal epididymis sperm
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• 8.31% of sperm have unsheathed flagella at their principal piece
|
|
|
• 41.43% of sperm have hairpin-like structures in their midpieces
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|
|
• fewer spermatozoa are seen in the caudal epididymis
|
|
|
• no progressive sperm are seen
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|
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• sperm show decreased motility and only 7.667% of sperm being motile compared to 82.33% of wild-type sperm
• mean path velocity and straight-line velocity of sperm are reduced
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