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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Thrap3tm1c(KOMP)Wtsi
targeted mutation 1c, Wellcome Trust Sanger Institute
MGI:7522740
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Thrap3tm1c(KOMP)Wtsi/Thrap3tm1c(KOMP)Wtsi
Speer6-ps1Tg(Alb-cre)21Mgn/Speer6-ps1+
involves: C57BL/6 * C57BL/6J * C57BL/6N * DBA MGI:7531058


Genotype
MGI:7531058
cn1
Allelic
Composition
Thrap3tm1c(KOMP)Wtsi/Thrap3tm1c(KOMP)Wtsi
Speer6-ps1Tg(Alb-cre)21Mgn/Speer6-ps1+
Genetic
Background
involves: C57BL/6 * C57BL/6J * C57BL/6N * DBA
Cell Lines EPD0814_5_G12
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Speer6-ps1Tg(Alb-cre)21Mgn mutation (6 available); any Speer6-ps1 mutation (4 available)
Thrap3tm1c(KOMP)Wtsi mutation (0 available); any Thrap3 mutation (83 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• mice fed a high-fat diet (HFD) for 12 weeks exhibit a significantly lower body weight than HFD-fed control mice
• however, body weight is normal under normal chow diet (NCD) conditions

homeostasis/metabolism
• livers from HFD-fed mice exhibit a significantly higher number of autophagic structures (autophagosomes/autolysosomes) than livers from diet-matched controls
• most of the increased LC3 puncta in FFA-treated hepatocytes colocalize with MitoTracker (a mitochondrial-specific dye), indicating enhanced mitophagy; PINK1 (a marker protein of mitophagy) is increased in the liver
• liver lysates from HFD-fed mice show increased expression of autophagosome proteins MAP1LC3A (LC3), ULK1 and SQSTM1 (p62)
• primary hepatocytes transfected with GFP-LC3 have more LC3 puncta per cell than control hepatocytes
• lysates from primary hepatocytes treated with bafilomycin A1 (BafA1) show significantly more LC3 than BafA1-treated control hepatocytes
• HFD-fed mice exhibit significantly lower blood glucose levels than diet-matched controls
• however, blood glucose levels are normal under NCD conditions
• HFD-fed mice exhibit significantly lower serum insulin levels than diet-matched controls
• however, serum insulin levels are normal under NCD conditions
• HFD-fed mice exhibit significantly lower serum cholesterol levels than diet-matched controls
• however, serum cholesterol levels are normal under NCD conditions
• HFD-fed mice exhibit significantly lower serum FFA levels than diet-matched controls
• however, serum FFA levels are normal under NCD conditions
• HFD-fed mice exhibit significantly lower serum TG levels than diet-matched controls
• however, serum TG levels are normal under NCD conditions
• HFD-fed mice exhibit significantly lower serum ALT levels than diet-matched controls
• however, serum ALT levels are normal under NCD conditions
• HFD-fed mice exhibit significantly lower serum AST levels than diet-matched controls
• however, serum AST levels are normal under NCD conditions
• HFD-fed mice show a significantly higher energy expenditure than diet-matched controls
• primary hepatocytes from HFD-fed mice show an improved oxygen consumption rate
• HFD-fed mice show a significantly higher oxygen consumption rate (VO2) during day and night
• however, no changes in locomotor activity or food intake are noted under HFD conditions
• HFD-fed mice exhibit improved glucose tolerance relative to diet-matched controls
• however, glucose tolerance is normal under NCD conditions
• however, insulin tolerance is normal under NCD conditions
• HFD-fed mice exhibit improved insulin sensitivity relative to diet-matched controls
• mice fed a HFD or a MCD diet exhibit significantly lower liver cholesterol levels than diet-matched controls
• however, liver cholesterol levels are normal under NCD conditions
• mice fed a HFD or a MCD diet exhibit significantly lower liver triglyceride levels than diet-matched controls
• however, liver triglyceride levels are normal under NCD conditions

liver/biliary system
• HFD-fed mice exhibit a significantly higher mtDNA level in the liver than diet-matched controls
• mice fed a HFD or a MCD diet exhibit significantly lower liver cholesterol levels than diet-matched controls
• however, liver cholesterol levels are normal under NCD conditions
• mice fed a HFD or a MCD diet exhibit significantly lower liver triglyceride levels than diet-matched controls
• however, liver triglyceride levels are normal under NCD conditions
• mice fed a HFD for 12 weeks exhibit attenuated hepatic steatosis, as indicated by a darker liver color and a significant reduction in liver size, liver weight, hepatic triglyceride and cholesterol level, nonalcoholic fatty liver disease (NAFLD) activity score (NAS), and fibrosis score relative to HFD-fed control mice
• mice fed a methionine/choline-deficient (MCD) diet for 4 weeks also show reduced hepatic steatosis with darker liver color and significantly lower hepatic triglyceride and cholesterol levels, NAS and fibrosis scores than MCD-fed control mice
• however, mice show no differences in liver size, weight or lipid accumulation when fed a normal chow diet (NCD)
• following insulin administration, both HFD-fed and NCD-fed mice show increased phosphorylation of the Ser473 residue of AKT in the liver relative to controls
• however, expression of fatty acid beta-oxidation- and lipogenesis-related genes is not significantly altered
• primary hepatocytes from HFD-fed mice show a significant increase in the extracellular acidification rate (ECAR)

cellular
• HFD-fed mice exhibit a significantly higher mtDNA level in the liver than diet-matched controls
• HFD-fed mice exhibit a significantly higher cristae volume density in the liver than diet-matched controls
• HFD-fed mice exhibit a significantly greater area of mitochondria in the liver than diet-matched controls
• livers from HFD-fed mice exhibit a significantly higher number of autophagic structures (autophagosomes/autolysosomes) than livers from diet-matched controls
• most of the increased LC3 puncta in FFA-treated hepatocytes colocalize with MitoTracker (a mitochondrial-specific dye), indicating enhanced mitophagy; PINK1 (a marker protein of mitophagy) is increased in the liver
• liver lysates from HFD-fed mice show increased expression of autophagosome proteins MAP1LC3A (LC3), ULK1 and SQSTM1 (p62)
• primary hepatocytes transfected with GFP-LC3 have more LC3 puncta per cell than control hepatocytes
• lysates from primary hepatocytes treated with bafilomycin A1 (BafA1) show significantly more LC3 than BafA1-treated control hepatocytes
• HFD-fed mice show significantly higher levels of mitochondrial respiratory chain complex proteins, such as succinate dehydrogenase complex iron sulfur subunit B (SDHB), cytochrome b-c1 complex subunit 2 (UQCR2), and ATP synthase subunit alpha (ATP5A) in the liver than diet-matched controls
• primary hepatocytes from HFD-fed mice show a significant increase in ATP production





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory